Gene expression profile analysis of an isogenic tumour metastasis model reveals a functional role for oncogene AF1Q in breast cancer metastasis | |
Li, Da-Qiang; Hou, Yi-Feng; Wu, Jiong; Chen, Yi; Lu, Jin-Song; Di, Gen-Hong; Ou, Zhou-Luo; Shen, Zhen-Zhou; Ding, Jian; Shao, Zhi-Min | |
刊名 | EUROPEAN JOURNAL OF CANCER |
2006-12 | |
卷号 | 42期号:18页码:3274-3286 |
关键词 | breast cancer metastasis gene expression profile ALL1-fused gene from chromosome 1q |
ISSN号 | 0959-8049 |
DOI | 10.1016/j.ejca.2006.07.008 |
文献子类 | Article |
英文摘要 | To study the molecular mechanisms underlying breast cancer metastasis, gene expression profile analysis was performed on two well-established breast cancer cell lines with high and low metastatic potentials: MDA-MB-435HM and MDA-MB-435LM. The analysis was conducted using cDNA microarrays containing 8000 genes. Of 60 differentially expressed genes, ALL1-fused gene from chromosome 1q (AF1Q), a putative oncogene not described previously in breast cancer, was identified and found to be over-expressed in MDA-MB-435HM cells compared with MDA-MB-435LM cells. The results indicate that AF1Q may play an important role in breast cancer metastasis. To test this hypothesis, we generated an AF1Q high-expression cell line by stable transfection of AF1Q cDNA into MDA-MB-435LM cells. Results showed that over-expression of AF1Q led to a marked increase in the invasive and metastatic potential of MDA-MB-435LM cells in vitro and in vivo, accompanied by the up-regulation of matrix metalloproteinase-2 (MMP-2), MMP-9, transcription factor Ets-1, and RhoC expression in both mRNA and protein levels. Consistent with this observation, reduced AF1Q expression in MDA-MB-435HM cells by small interfering RNA (siRNA) resulted in a significant decrease in the invasive potential of MDA-MB-435HM cells in vitro and in the protein expression of MMP-2, MMP-9, Ets-1, and RhoC, compared with either parental or non-silencing control cells. These data provide functional evidence that oncogene AF1Q may be a novel mediator of metastasis promotion in human breast cancer through regulation of the MMP pathway and RhoC expression. (c) 2006 Elsevier Ltd. All rights reserved. |
WOS关键词 | MAMMARY EPITHELIAL-CELLS ; IN-VIVO ; TRANSCRIPTION FACTOR ; CARCINOMA-CELLS ; ACUTE-LEUKEMIA ; PROGRESSION ; RHOC ; INVASION ; METALLOPROTEINASES ; CLASSIFICATION |
WOS研究方向 | Oncology |
语种 | 英语 |
出版者 | ELSEVIER SCI LTD |
WOS记录号 | WOS:000243304900035 |
内容类型 | 期刊论文 |
源URL | [http://119.78.100.183/handle/2S10ELR8/273433] |
专题 | 新药研究国家重点实验室 中科院受体结构与功能重点实验室 |
通讯作者 | Shao, Zhi-Min |
作者单位 | 1.Fudan Univ, Canc Hosp, Inst Canc, Dept Breast Surg,Breast Canc Inst, Shanghai 200032, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Div Anti Tumor Pharmacol, Shanghai 201203, Peoples R China |
推荐引用方式 GB/T 7714 | Li, Da-Qiang,Hou, Yi-Feng,Wu, Jiong,et al. Gene expression profile analysis of an isogenic tumour metastasis model reveals a functional role for oncogene AF1Q in breast cancer metastasis[J]. EUROPEAN JOURNAL OF CANCER,2006,42(18):3274-3286. |
APA | Li, Da-Qiang.,Hou, Yi-Feng.,Wu, Jiong.,Chen, Yi.,Lu, Jin-Song.,...&Shao, Zhi-Min.(2006).Gene expression profile analysis of an isogenic tumour metastasis model reveals a functional role for oncogene AF1Q in breast cancer metastasis.EUROPEAN JOURNAL OF CANCER,42(18),3274-3286. |
MLA | Li, Da-Qiang,et al."Gene expression profile analysis of an isogenic tumour metastasis model reveals a functional role for oncogene AF1Q in breast cancer metastasis".EUROPEAN JOURNAL OF CANCER 42.18(2006):3274-3286. |
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