Structure elucidation and sulfated derivatives preparation of two alpha-D-glucans from Gastrodia elata Bl. and their anti-dengue virus bioactivities

doi:10.1016/j.carres.2007.06.021

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 药理学第一研究室

	Structure elucidation and sulfated derivatives preparation of two alpha-D-glucans from Gastrodia elata Bl. and their anti-dengue virus bioactivities
	Qiu, Hong ; Tang, Wei; Tong, Xiankun; Ding, Kan; Zuo, Jianping
刊名	CARBOHYDRATE RESEARCH
	2007-11-05
卷号	342 期号:15 页码:2230-2236
关键词	Gastrodia elata alpha-D-glucan dengue virus sulfated polysaccharide
ISSN号	0008-6215
DOI	10.1016/j.carres.2007.06.021
文献子类	Article
英文摘要	The structures of two glucans, WGEW and AGEW, isolated from Gastrodia elata Bl. were elucidated using monosaccharide composition analysis by gas chromatography (GC), methylation analysis by gas chromatography-mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR) spectroscopy. Their structures were deduced as an alpha-D-(1 --> 4)-glucan with an alpha-(1 --> 4) linked branch attached to O-6 branch points with different branch degrees. Their sulfate derivatives with distinct degrees of substitution (DS) were prepared. The substitution position was assigned to O-6 according to the C-13 NMR spectra. All sulfated derivatives showed strong anti-dengue virus bioactivities. The structure-activity relationships (SAR) between the polysaccharides and their sulfated derivatives were also investigated. Results showed that the higher the DS is, the more potent the impact on the dengue virus infection would be. (C) 2007 Elsevier Ltd. All rights reserved.
WOS关键词	COLORIMETRIC ASSAY ; HEPARAN-SULFATE ; POLYSACCHARIDES ; CELL ; SEROTYPE ; AGENTS
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000249859000009
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/273115]
专题	药理学第一研究室中科院受体结构与功能重点实验室新药研究国家重点实验室
通讯作者	Ding, Kan
作者单位	1.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Materia Med, Glycobiol & Glycochem Lab,State Key Lab Drug Res, Shanghai 201203, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Materia Med, Lab Immunopharmacol,State Key Lab Drug Res, Shanghai 201203, Peoples R China 3.Shanghai Univ Tradit Chinese Med, Lab Immunol & Virol, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Qiu, Hong,Tang, Wei,Tong, Xiankun,et al. Structure elucidation and sulfated derivatives preparation of two alpha-D-glucans from Gastrodia elata Bl. and their anti-dengue virus bioactivities[J]. CARBOHYDRATE RESEARCH,2007,342(15):2230-2236.
APA	Qiu, Hong,Tang, Wei,Tong, Xiankun,Ding, Kan,&Zuo, Jianping.(2007).Structure elucidation and sulfated derivatives preparation of two alpha-D-glucans from Gastrodia elata Bl. and their anti-dengue virus bioactivities.CARBOHYDRATE RESEARCH,342(15),2230-2236.
MLA	Qiu, Hong,et al."Structure elucidation and sulfated derivatives preparation of two alpha-D-glucans from Gastrodia elata Bl. and their anti-dengue virus bioactivities".CARBOHYDRATE RESEARCH 342.15(2007):2230-2236.