Improved and simplified LC-ESI-MS/MS method for homocysteine determination in human plasma: Application to the study of cardiovascular diseases

doi:10.1016/j.jchromb.2008.06.003

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	Improved and simplified LC-ESI-MS/MS method for homocysteine determination in human plasma: Application to the study of cardiovascular diseases
	Li, Shuijun 1,3; Jia, Jingying 1; Liu, Gangyi 1; Wang, Wei 2; Cai, Yongbao 2; Wang, Yiping3 ; Yu, Chen 1
刊名	JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
	2008-07-01
卷号	870 期号:1 页码:63-67
关键词	homocysteine LC-ESI-MS/MS cardiovascular disease method comparison
ISSN号	1570-0232
DOI	10.1016/j.jchromb.2008.06.003
文献子类	Article
英文摘要	A liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) method was developed and validated for the determination of human plasma homocysteine (Hcy), an important independent risk factor for cardiovascular disease, with a simplified sample pretreatment procedure and a zero blank free of endogenous Hcy for calibrator/QC preparation. Following protein precipitation, chromatographic separation was performed on Hypersil Aquasil C-18 column (50 mm x 2.1 mm, 5 mu m, Thermo) using mobile phase of aqueous 10% methanol containing 0.02% formic acid at 0.25 mL/min. Hcy and deuterated internal standard were detected in the multiple reaction monitoring mode with precursor to product ion transitions of m/z 136.1/90.0 and 140.1/94.0, respectively. The retention time was 1.2 min, and the total run time was 2 min per injection. A streamlined three-point calibration curve and one-point QC was used. Excellent linearity was observed with correlation coefficient (r) > 0.99. The intra- and inter-batch were <= 3.24% and <= 4.04%, and accuracy was within +/- 10%. Method comparison between the proposed method (y) and FPIA assay (x) demonstrated a correlation equation of y = 1.003x + 0.4318 (r = 0.9589). The developed method, improved for automation with cost-effective reagents, was proven to be suitable for high-throughput quantitative determination of Hcy in clinical practice by successfully applying it to the cardiovascular disease study. (C) 2008 Elsevier B.V. All rights reserved.
WOS关键词	TANDEM MASS-SPECTROMETRY ; INDUCED FLUORESCENCE DETECTION ; LIQUID-CHROMATOGRAPHY ; CAPILLARY-ELECTROPHORESIS ; METHYLMALONIC ACID ; ASSAY ; PERFORMANCE ; CYSTEINE ; URINE ; SERUM
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000257835000011
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/272880]
专题	药理学第一研究室中科院受体结构与功能重点实验室新药研究国家重点实验室
通讯作者	Yu, Chen
作者单位	1.Shanghai Xuhui Cent Hosp, Cent Lab, Shanghai 200031, Peoples R China; 2.Shanghai Xuhui Cent Hosp, Dept Cardiol, Shanghai 200031, Peoples R China; 3.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Li, Shuijun,Jia, Jingying,Liu, Gangyi,et al. Improved and simplified LC-ESI-MS/MS method for homocysteine determination in human plasma: Application to the study of cardiovascular diseases[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2008,870(1):63-67.
APA	Li, Shuijun.,Jia, Jingying.,Liu, Gangyi.,Wang, Wei.,Cai, Yongbao.,...&Yu, Chen.(2008).Improved and simplified LC-ESI-MS/MS method for homocysteine determination in human plasma: Application to the study of cardiovascular diseases.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,870(1),63-67.
MLA	Li, Shuijun,et al."Improved and simplified LC-ESI-MS/MS method for homocysteine determination in human plasma: Application to the study of cardiovascular diseases".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 870.1(2008):63-67.