Huperzine A attenuates mitochondrial dysfunction after middle cerebral artery occlusion in rats

doi:10.1002/jnr.21681

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 药理学第二研究室

	Huperzine A attenuates mitochondrial dysfunction after middle cerebral artery occlusion in rats
	Zheng, Chun Yan; Zhang, Hai Yan; Tang, Xi Can
刊名	JOURNAL OF NEUROSCIENCE RESEARCH
	2008-08-15
卷号	86 期号:11 页码:2432-2440
关键词	acetylcholinesterase inhibitor calcium huperzine A ischemia mitochondria
ISSN号	0360-4012
DOI	10.1002/jnr.21681
文献子类	Article
英文摘要	Mitochondrial dysfunction has been proved to contribute to ischemia-induced brain damage. In this study, which used a rat middle cerebral artery occlusion (MCAO) model, the protective effects of huperzine A (HupA) against mitochondrial dysfunction and brain damage were investigated. MCAO for 45 min followed by 4 hr of reperfusion significantly impaired the activities of mitochondrial respiratory chain enzymes (complex I, complex II-III, and complex IV) and a-ketoglutarate dehydrogenase, increased the production of reactive oxygen species (ROS), and induced mitochondrial swelling. Pretreatment of HupA at 0.1 mg/kg significantly preserved respiratory chain enzyme activities, decreased ROS production, and attenuated mitochondrial swelling. It could also significantly attenuate the neurological deficits (after 4 or 24 hr reperfusion) and reduce infarct volumes (after 24 hr reperfusion). Moreover, HupA protected isolated nonsynaptosomal mitochondria from calcium-induced damage in vitro by preserving mitochondrial membrane potential and decreasing ROS production. Overall, the present study indicates that HupA can ameliorate MCAO-induced mitochondrial dysfunction, and this might partially contribute to its protective effect on brain damage after 24 hr of reperfusion. (c) 2008 Wiley-Liss, Inc.
资助项目	National Natural Science Foundation of China[30572169] ; Ministry of Science and Technology of China[2004CB518907]
WOS关键词	KETOGLUTARATE DEHYDROGENASE COMPLEX ; OXYGEN-GLUCOSE DEPRIVATION ; TRANSIENT FOCAL ISCHEMIA ; INDUCED OXIDATIVE INJURY ; DELAYED NEURONAL DEATH ; COGNITIVE DEFICITS ; BRAIN MITOCHONDRIA ; CYTOCHROME-C ; BLOOD-FLOW ; CELL-DEATH
WOS研究方向	Neurosciences & Neurology
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000258478100008
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/272836]
专题	药理学第二研究室中科院受体结构与功能重点实验室新药研究国家重点实验室院士及顾问专家
通讯作者	Zhang, Hai Yan
作者单位	Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Zheng, Chun Yan,Zhang, Hai Yan,Tang, Xi Can. Huperzine A attenuates mitochondrial dysfunction after middle cerebral artery occlusion in rats[J]. JOURNAL OF NEUROSCIENCE RESEARCH,2008,86(11):2432-2440.
APA	Zheng, Chun Yan,Zhang, Hai Yan,&Tang, Xi Can.(2008).Huperzine A attenuates mitochondrial dysfunction after middle cerebral artery occlusion in rats.JOURNAL OF NEUROSCIENCE RESEARCH,86(11),2432-2440.
MLA	Zheng, Chun Yan,et al."Huperzine A attenuates mitochondrial dysfunction after middle cerebral artery occlusion in rats".JOURNAL OF NEUROSCIENCE RESEARCH 86.11(2008):2432-2440.