GPCR Activation and Endocytosis Induced by a 2D Material Agonist

doi:10.1021/acsami.7b02754

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	GPCR Activation and Endocytosis Induced by a 2D Material Agonist
	Dou, Wei-Tao 1,2; Kong, Ya 3,4; He, Xiao-Peng 1,2; Chen, Guo-Rong 1,2; Zang, Yi3,4 ; Li, Jia3,4 ; Tian, He 1,2
刊名	ACS APPLIED MATERIALS & INTERFACES
	2017-05-03
卷号	9 期号:17 页码:14709-14715
关键词	2D material G protein-coupled receptor ligand peptide fluorescence imaging photodynamic therapy endocytosis
ISSN号	1944-8244
DOI	10.1021/acsami.7b02754
文献子类	Article
英文摘要	Agonist-induced activation and endocytosis of G protein-coupled receptors (GPCRs) are crucial for a number of physiological and pathological processes. However, tools that are available for probing GPCR endocytosis have been insufficient. Here, we developed,a two-dimensional (212) material agonist by supramolecular self-assembly between an endogenous agonist of K-opioid receptor (KOR) and 21) molybdenum disulfide. The 2D material agonist has proven to, be amenable for eliciting GPCR activation and endocytosis in cells stably expressing KOR rather than in those without KOR expression. Using super-resolution Microscopy, we also Show that the 2D material agonist colocalizes well with GFP-fused KOR intracellularly. Further, the endocytosed 2D,material agonist can selectively produce reactive oxygen species in cells that overly express KOR, as controlled by light irradiation.
资助项目	973 project[2013CB733700] ; Natural Science Foundation of China[21572058] ; Natural Science Foundation of China[21576088] ; Natural Science Foundation of China[81673489] ; Shanghai Science and Technology Development Funds[16430724100] ; Fundamental Research Funds for Central Universities[222201717003] ; Shanghai Rising-Star Program[16QA1401400]
WOS关键词	PROTEIN-COUPLED RECEPTORS ; TRANSITION-METAL DICHALCOGENIDES ; KAPPA-OPIOID RECEPTOR ; PEGYLATED MOS2 NANOSHEETS ; BOTTOM-UP SYNTHESIS ; DRUG DISCOVERY ; CANCER-THERAPY ; BIOMEDICAL APPLICATIONS ; PHOTODYNAMIC THERAPY ; DYNORPHIN PEPTIDES
WOS研究方向	Science & Technology - Other Topics ; Materials Science
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000400802700018
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/272671]
专题	国家新药筛选中心中科院受体结构与功能重点实验室新药研究国家重点实验室药物安全性评价中心
通讯作者	He, Xiao-Peng; Zang, Yi; Li, Jia
作者单位	1.East China Univ Sci & Technol, Key Lab Adv Mat, Sch Chem & Mol Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China; 2.East China Univ Sci & Technol, Inst Fine Chem, Sch Chem & Mol Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China; 3.Chinese Acad Sci, Natl Ctr Drug Screening, State Key Lab Drug Res, Shanghai Inst Mat Med, 189 Guo Shoujing Rd, Shanghai 201203, Peoples R China; 4.UCAS, 19A,Yuquan Rd, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Dou, Wei-Tao,Kong, Ya,He, Xiao-Peng,et al. GPCR Activation and Endocytosis Induced by a 2D Material Agonist[J]. ACS APPLIED MATERIALS & INTERFACES,2017,9(17):14709-14715.
APA	Dou, Wei-Tao.,Kong, Ya.,He, Xiao-Peng.,Chen, Guo-Rong.,Zang, Yi.,...&Tian, He.(2017).GPCR Activation and Endocytosis Induced by a 2D Material Agonist.ACS APPLIED MATERIALS & INTERFACES,9(17),14709-14715.
MLA	Dou, Wei-Tao,et al."GPCR Activation and Endocytosis Induced by a 2D Material Agonist".ACS APPLIED MATERIALS & INTERFACES 9.17(2017):14709-14715.