Label-free brainwide visualization of senile plaque using cryo-micro-optical sectioning tomography

doi:10.1364/OL.42.004247

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	Label-free brainwide visualization of senile plaque using cryo-micro-optical sectioning tomography
	Luo, Yilin 2,3; Wang, Anle 1,2,3; Liu, Mengmeng 2,3; Lei, Tian 2,3; Zhang, Xiaochuan 4; Gao, Zhaobing4 ; Jiang, Hualiang4 ; Gong, Hui 2,3; Yuan, Jing 2,3
刊名	OPTICS LETTERS
	2017-11-01
卷号	42 期号:21 页码:4247-4250
ISSN号	0146-9592
DOI	10.1364/OL.42.004247
文献子类	Article
英文摘要	Optical visualization of pathological changes in Alzheimer's disease (AD) can facilitate exploration of disease mechanisms and treatments. However, existing optical imaging methods have limitations on mapping pathological evolution in the whole mouse brain. Previous research indicated endogenous fluorescence contrast of senile plaques. Therefore, we develop cryo-micro-optical sectioning tomography (cryoMOST) to capture intrinsic fluorescence distribution of senile plaques at a micrometer-level resolution in the whole brain. Validation using immunofluorescence demonstrates the capacity of cryo-MOST to visualize and distinguish senile plaques with competent sensitivity and spatial resolution. Compared with imaging in room temperature, cryoMOST provides better signal intensity and signal-to-noise ratio. Using cryo-MOST, we obtained whole-brain coronal distribution of senile plaques in a transgenic mouse without exogenous dye. Capable of label-free brainwide visualization of Alzheimer's pathology, cryo-MOST may be potentially useful for understanding neurodegenerative disease mechanisms and evaluating drug efficacy. (C) 2017 Optical Society of America
资助项目	Ministry of Science and Technology of the People's Republic of China (MOST)[2012YQ03026001] ; Ministry of Science and Technology of the People's Republic of China (MOST)[2012YQ03026010] ; National Natural Science Foundation of China (NSFC)[61421064] ; Huazhong University of Science and Technology (HUST) (Director Fund of WNLO)[00000000]
WOS关键词	ALZHEIMERS-DISEASE ; AMYLOID PLAQUES ; MOUSE MODEL
WOS研究方向	Optics
语种	英语
出版者	OPTICAL SOC AMER
WOS记录号	WOS:000414097200003
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/272432]
专题	神经药理学研究国际科学家工作站中科院受体结构与功能重点实验室新药研究国家重点实验室药物发现与设计中心
通讯作者	Yuan, Jing
作者单位	1.Air Force Early Warning Acad, Wuhan 430017, Hubei, Peoples R China; 2.Huazhong Univ Sci & Technol, Wuhan Natl Lab Optoelect, Britton Chance Ctr Biomed Photon, Wuhan 430074, Hubei, Peoples R China; 3.Huazhong Univ Sci & Technol, Sch Engn Sci, Collaborat Innovat Ctr Biomed Engn, MoE Key Lab Biomed Photon, Wuhan 430074, Hubei, Peoples R China; 4.Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, State Key Lab Drug Res, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Luo, Yilin,Wang, Anle,Liu, Mengmeng,et al. Label-free brainwide visualization of senile plaque using cryo-micro-optical sectioning tomography[J]. OPTICS LETTERS,2017,42(21):4247-4250.
APA	Luo, Yilin.,Wang, Anle.,Liu, Mengmeng.,Lei, Tian.,Zhang, Xiaochuan.,...&Yuan, Jing.(2017).Label-free brainwide visualization of senile plaque using cryo-micro-optical sectioning tomography.OPTICS LETTERS,42(21),4247-4250.
MLA	Luo, Yilin,et al."Label-free brainwide visualization of senile plaque using cryo-micro-optical sectioning tomography".OPTICS LETTERS 42.21(2017):4247-4250.