Improved Chromatographic Fingerprinting Combined with Multi-components Quantitative Analysis for Quality Evaluation of Penthorum chinense by UHPLC-DAD

	Improved Chromatographic Fingerprinting Combined with Multi-components Quantitative Analysis for Quality Evaluation of Penthorum chinense by UHPLC-DAD
	Deng, Wangping 3; Xu, Tongtong 1; Yang, Min 2; Cui, Yajun 3; Guo, De-an2
刊名	NATURAL PRODUCT COMMUNICATIONS
	2015-01
卷号	10 期号:1 页码:71-76
关键词	Penthoruni chinense Pursh UHPLC Fingerprint Multi-components quantitative analysis Method validation Similarity PCA
ISSN号	1934-578X
文献子类	Article
英文摘要	A high performance liquid chromatographic (HPLC) fingerprint is commonly used for quality consistency evaluation of herbal medicines. Recently, an improved chromatographic technique resulted in ultra high performance liquid chromatography (UHPLC), which could provide higher resolution in less time under higher pressure using finer particles (less than 2 mu m) of stationary phase. A simple and sensitive method was developed and validated for fingerprint analysis of Penthorum chinense Pursh (PC), with the simultaneous determination of seven components using UPLC coupled with a diode-array detector (DAD). It took less than 20 min for analysis of one sample. Both similarity analysis and principle components analysis (PCA) were employed to evaluate the quality consistency of 17 sample batches. The analysis was performed on a Waters ACQUITY UPLC HSS T3 (2.1 x 150 mm, 1.7 gm) column, which was maintained at 45 degrees C and the eluents were monitored with DAD at 270 nm. A gradient elution with acetonitrile and water containing 0.075% phosphoric acid was used. The solvent flow rate was 0.4 mL/min. Standard calibration curves showed good linear behavior (R-2>0.9994) in the range of 0.20-337.05 mu g/mL. Acceptable repeatability (RSD<0.61%), reproducibility (RSD<2.72%), stability (RSD<1.59%) and recovery in the range of 94.7%-102.9% were obtained (precision and accuracy). The validated method was successfully applied to evaluate the quality of 21 samples of PC.
资助项目	National Natural Science Foundation of China[81373965] ; Twelfth Five-Year National Science & Technology Support Program[2012BAI29B06] ; Chinese Academy of Sciences[KSCX2-YWR-166] ; National Science & Technology Major Project 'Key New Drug Creation and Manufacturing program' of the People's Republic of China[2011ZX09307-002-03]
WOS关键词	GALLIC ACID ; LIVER
WOS研究方向	Pharmacology & Pharmacy ; Food Science & Technology
语种	英语
出版者	NATURAL PRODUCTS INC
WOS记录号	WOS:000348825700017
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/276764]
专题	中国科学院上海药物研究所
通讯作者	Yang, Min
作者单位	1.Changchun Univ Chinese Med, Changchun 130117, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Natl Engn Lab TCM Standardizat Technol, Shanghai 201203, Peoples R China; 3.Shanghai Univ Tradit Chinese Med, Sch Chinese Mat Med, Shanghai 201203, Peoples R China;
推荐引用方式 GB/T 7714	Deng, Wangping,Xu, Tongtong,Yang, Min,et al. Improved Chromatographic Fingerprinting Combined with Multi-components Quantitative Analysis for Quality Evaluation of Penthorum chinense by UHPLC-DAD[J]. NATURAL PRODUCT COMMUNICATIONS,2015,10(1):71-76.
APA	Deng, Wangping,Xu, Tongtong,Yang, Min,Cui, Yajun,&Guo, De-an.(2015).Improved Chromatographic Fingerprinting Combined with Multi-components Quantitative Analysis for Quality Evaluation of Penthorum chinense by UHPLC-DAD.NATURAL PRODUCT COMMUNICATIONS,10(1),71-76.
MLA	Deng, Wangping,et al."Improved Chromatographic Fingerprinting Combined with Multi-components Quantitative Analysis for Quality Evaluation of Penthorum chinense by UHPLC-DAD".NATURAL PRODUCT COMMUNICATIONS 10.1(2015):71-76.