Cellulosimicrobium cellulans strain E4-5 enzymatic hydrolysis of curdlan for production of (1 -> 3)-linked beta-D-glucan oligosaccharides

doi:10.1016/j.carbpol.2015.08.019

	Cellulosimicrobium cellulans strain E4-5 enzymatic hydrolysis of curdlan for production of (1 -> 3)-linked beta-D-glucan oligosaccharides
	Fu, Yunbin 2,3; Cheng, Likun 2; Meng, Yanyu 1,2; Li, Shuguang 2; Zhao, Xiaoming 2; Du, Yuguang 2,4; Yin, Heng 2
刊名	CARBOHYDRATE POLYMERS
	2015-12-10
卷号	134 页码:740-744
关键词	Cellulosimicrobium cellulans Enzymatic degradation Curdlan High-set gel Alkali-neutralization treatment (1 -> 3)-Linked beta-D-glucan oligosaccharides
ISSN号	0144-8617
DOI	10.1016/j.carbpol.2015.08.019
文献子类	Article
英文摘要	In order to find an efficient enzymatic tool for curdlan degradation to produce (1 -> 3)-linked beta-D-glucan oligosaccharides, strain E4-5 (registration number JN089883, Genbank) was isolated from seaside soil. The 16S rRNA gene sequencing classified it as Cellulosimicrobium cellulans. It was the first reported microorganism that succeeded in degrading high-set heated curdlan blocks. The ferments of strain E4-5 also showed good degradation effects on laminaran and alkali-neutralized curdlan. Due to the products with less amount of glucose, it was assumed that endo-1,3-beta-glucanases of strain E4-5 had a greater hydrolyzing effect than exo-1,3-beta-glucanases. This indicated that strain E4-5 was a promising microorganism to hydrolyze (1 -> 3)-linked beta-D-glucan. Moreover, alkali-neutralization pretreatment was effective for promoting a more diversified degree of polymerization (DP) of (1 -> 3)-linked beta-D-glucan oligosaccharides under enzymatic hydrolysis and will pave the way for making full use of curdlan for production of glucan oligosaccharides. (C) 2015 Elsevier Ltd. All rights reserved.
资助项目	Hi-Tech Research and Development Program of China[2011AA10A205] ; Hi-Tech Research and Development Program of China[2012AA021501] ; National Natural Science Foundation of China[31370811]
WOS关键词	ALPHA-AMYLASE ; RESISTANCE ; GELATION
WOS研究方向	Chemistry ; Polymer Science
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000364255800089
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/276285]
专题	中国科学院上海药物研究所
通讯作者	Du, Yuguang
作者单位	1.Henan Agr Univ, Coll Sci, Zhengzhou 450002, Henan, Peoples R China; 2.Chinese Acad Sci, Dalian Inst Chem Phys, Nat Prod & Glycobiotechnol Grp 1805, Dalian 116023, Liaoning, Peoples R China; 3.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China; 4.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Fu, Yunbin,Cheng, Likun,Meng, Yanyu,et al. Cellulosimicrobium cellulans strain E4-5 enzymatic hydrolysis of curdlan for production of (1 -> 3)-linked beta-D-glucan oligosaccharides[J]. CARBOHYDRATE POLYMERS,2015,134:740-744.
APA	Fu, Yunbin.,Cheng, Likun.,Meng, Yanyu.,Li, Shuguang.,Zhao, Xiaoming.,...&Yin, Heng.(2015).Cellulosimicrobium cellulans strain E4-5 enzymatic hydrolysis of curdlan for production of (1 -> 3)-linked beta-D-glucan oligosaccharides.CARBOHYDRATE POLYMERS,134,740-744.
MLA	Fu, Yunbin,et al."Cellulosimicrobium cellulans strain E4-5 enzymatic hydrolysis of curdlan for production of (1 -> 3)-linked beta-D-glucan oligosaccharides".CARBOHYDRATE POLYMERS 134(2015):740-744.