Tetrahydroberberine inhibits acetylcholine-induced K+ current in acutely dissociated rat hippocampal CA1 pyramidal neurons

doi:10.1016/S0304-3940(97)13356-0

	Tetrahydroberberine inhibits acetylcholine-induced K+ current in acutely dissociated rat hippocampal CA1 pyramidal neurons
	Wu, J ; Jin, GZ
刊名	NEUROSCIENCE LETTERS
	1997-01-31
卷号	222 期号:2 页码:115-118
关键词	tetrahydroberberine acetylcholine K+ current rat hippocampus dissociated neurons patch-clamp
ISSN号	0304-3940
DOI	10.1016/S0304-3940(97)13356-0
文献子类	Article
英文摘要	The effects of a novel chemical type of dopamine receptor antagonist, the tetrahydroprotoberberine analogs (THPBs), on acetylcholine (ACh)-induced current were studied in freshly dissociated pyramidal neurons from rat hippocampal CA1 area using the nystatin perforated patch-clamp recording technique. Under voltage clamp conditions, the ACh-induced outward current (I-ACh) is sensitive to the muscarinic receptor antagonist, atropine and the K+ channel blocker, TEA. The reversal potential of I-ACh(-84.1+/-0.8 mV) is close to the K+ equilibrium potential, indicating that the I-ACh is mediated by a muscarinic receptor, and is carried mainly by K+. Tetrahydroberberine (THB) markedly reduced the I-ACh while its chemical analogs, l-stepholidine (l-SPD) or l-tetrahydropalmatine (l-THP), had little effect on the I-ACh. The half-maximal inhibitory concentration (IC50) of THB was 1.3x10(-5) M for a 10(-5) M ACh-induced I-ACh. THB suppressed the maximum of the ACh concentration-response curve without shifting the Hill coefficient, indicating a non-competitive inhibition. It is concluded that THE non-competitively inhibits the ACh-induced K+ current in a concentration-dependent manner, and that this inhibitory effect provides further evidence that THE plays its pharmacological roles in the central nervous system by effects other than through blockade of dopamine receptors. (C) 1997 Elsevier Science Ireland Ltd.
WOS关键词	L-TETRAHYDROPALMATINE ; L-STEPHOLIDINE ; DOPAMINE ; TETRAHYDROPROTOBERBERINES ; (-)-STEPHOLIDINE ; STRIATUM ; ARTERY ; CELLS
WOS研究方向	Neurosciences & Neurology
语种	英语
出版者	ELSEVIER SCI IRELAND LTD
WOS记录号	WOS:A1997WH24700012
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/274932]
专题	中国科学院上海药物研究所
作者单位	1.UNIV NEW MEXICO,SCH MED,DEPT NEUROL,ALBUQUERQUE,NM 87131 2.CHINESE ACAD SCI,SHANGHAI INST MAT MED,SHANGHAI 20031,PEOPLES R CHINA
推荐引用方式 GB/T 7714	Wu, J,Jin, GZ. Tetrahydroberberine inhibits acetylcholine-induced K+ current in acutely dissociated rat hippocampal CA1 pyramidal neurons[J]. NEUROSCIENCE LETTERS,1997,222(2):115-118.
APA	Wu, J,&Jin, GZ.(1997).Tetrahydroberberine inhibits acetylcholine-induced K+ current in acutely dissociated rat hippocampal CA1 pyramidal neurons.NEUROSCIENCE LETTERS,222(2),115-118.
MLA	Wu, J,et al."Tetrahydroberberine inhibits acetylcholine-induced K+ current in acutely dissociated rat hippocampal CA1 pyramidal neurons".NEUROSCIENCE LETTERS 222.2(1997):115-118.