Aluminum-induced apoptosis in cultured astrocytes and its effect on calcium homeostasis

doi:10.1016/S0006-8993(00)03057-2

	Aluminum-induced apoptosis in cultured astrocytes and its effect on calcium homeostasis
	Guo, GW ; Liang, YX
刊名	BRAIN RESEARCH
	2001-01-12
卷号	888 期号:2 页码:221-226
关键词	aluminum apoptosis calcium astrocytes cell cycle
ISSN号	0006-8993
DOI	10.1016/S0006-8993(00)03057-2
文献子类	Article
英文摘要	Aluminum exposure and apoptotic cell death has been implicated in several neurodegenerative conditions including Alzheimer's disease. In this study, we use cultured astrocytes to investigate the ability of aluminum to induce the apoptosis of astrocytes. The proportion of apoptotic cells and cell cycle distribution were determined by how cytometric analysis. Our results showed that exposure to aluminum at low levels (100 and 200 muM) for up to 6 days did not result in the apoptosis of astrocytes, and a dramatic blockage of apoptotic cells was found at 200 muM aluminum. However, at 400 muM, aluminum markedly induced the apoptosis of astrocytes, which was associated with a significant change in cell cycle distribution characterized by increase of G2/M phase cells (128%). Measurements of intracellular Ca2+ concentration using the fluorescent calcium indicator dye Fluo-3 demonstrated a significant increase in the levels of intracellular calcium after aluminum treatment. However, no differences were observed among aluminum-treated groups. These findings suggest that aluminum induce and block selectively the apoptosis of astrocytes, which depend upon the concentrations of aluminum. Increased intracellular Ca2+ may not be the primary mechanism of aluminum-mediated apoptotic cell death. (C) 2001 Elsevier Science B.V. All rights reserved.
WOS关键词	AMYOTROPHIC LATERAL SCLEROSIS ; CELL-DEATH ; NEURONAL DEATH ; ALZHEIMERS-DISEASE ; GLUTAMATE ; DEPRIVATION ; CYCLE ; CA2+ ; ACCUMULATION ; EXPRESSION
WOS研究方向	Neurosciences & Neurology
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000166427900004
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/274527]
专题	中国科学院上海药物研究所
通讯作者	Guo, GW
作者单位	1.Shanghai Med Univ, Dept Occupat Hlth, Shanghai 200032, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Dept Pharmacol 2, Shanghai 200031, Peoples R China
推荐引用方式 GB/T 7714	Guo, GW,Liang, YX. Aluminum-induced apoptosis in cultured astrocytes and its effect on calcium homeostasis[J]. BRAIN RESEARCH,2001,888(2):221-226.
APA	Guo, GW,&Liang, YX.(2001).Aluminum-induced apoptosis in cultured astrocytes and its effect on calcium homeostasis.BRAIN RESEARCH,888(2),221-226.
MLA	Guo, GW,et al."Aluminum-induced apoptosis in cultured astrocytes and its effect on calcium homeostasis".BRAIN RESEARCH 888.2(2001):221-226.