Effect of dopamine depletion on DARPP-32 protein in ischemic rat striatum

	Effect of dopamine depletion on DARPP-32 protein in ischemic rat striatum
	Sun, YF ; Tang, FM ; Ding, YM ; Chen, YT; Zhang, GY ; Jin, GZ
刊名	ACTA PHARMACOLOGICA SINICA
	2001-03
卷号	22 期号:3 页码:243-248
关键词	dopamine corpus striatum brain ischemia immunohistochemistry in situ hybridization
ISSN号	1671-4083
文献子类	Article
英文摘要	AIM: To study the effects of dopamine depletion on the phosphorylation level, intracellular distribution, and mRNA expression of DARPP-32 in the ischemic striatum and to elucidate the mechanisms underlying the ischemic injury. METHODS: A complex model of SN lesioning with 6-OHDA to deplete dopamine and four vessels occlusion for inducing forebrain ischemia was constructed in rats. DARPP-32 was investigated with autoradiogram, immunohistochemistry and in situ hybridization. RE SUETS: The [P-32]phosphate incorporation of DARPP-32 was reduced in vitro following ischemia. However, the [P-32] phosphate incorporation, the numbers of positive neurons, and mRNA expression of DARPP-32 were increased in SN lesioning plus ischemic rats with denervated striatum. CONCLUSION: Dopamine depletion reduced the DARPP-32 phosphorylation in vivo following ischemia, and protected DARPP-32 immunoreactivity and mRNA expression level against the reduction induced by ischemia.
WOS关键词	CEREBRAL-ISCHEMIA ; NEURONS ; PHOSPHOPROTEIN ; RECEPTORS
WOS研究方向	Chemistry ; Pharmacology & Pharmacy
语种	英语
CSCD记录号	CSCD:515788
出版者	ACTA PHARMACOLOGICA SINICA
WOS记录号	WOS:000167403800008
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/274512]
专题	中国科学院上海药物研究所
通讯作者	Tang, FM
作者单位	1.Xuzhou Med Coll, Res Ctr Biochem & Mol Biol, Xuzhou 221002, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China
推荐引用方式 GB/T 7714	Sun, YF,Tang, FM,Ding, YM,et al. Effect of dopamine depletion on DARPP-32 protein in ischemic rat striatum[J]. ACTA PHARMACOLOGICA SINICA,2001,22(3):243-248.
APA	Sun, YF,Tang, FM,Ding, YM,Chen, YT,Zhang, GY,&Jin, GZ.(2001).Effect of dopamine depletion on DARPP-32 protein in ischemic rat striatum.ACTA PHARMACOLOGICA SINICA,22(3),243-248.
MLA	Sun, YF,et al."Effect of dopamine depletion on DARPP-32 protein in ischemic rat striatum".ACTA PHARMACOLOGICA SINICA 22.3(2001):243-248.