Jolkinolide B induces neuroendocrine differentiation of human prostate LNCaP cancer cell line

doi:10.1016/S0006-2952(01)00938-8

	Jolkinolide B induces neuroendocrine differentiation of human prostate LNCaP cancer cell line
	Liu, WK ; Ho, JCK ; Qin, GW ; Che, CT
刊名	BIOCHEMICAL PHARMACOLOGY
	2002-03-01
卷号	63 期号:5 页码:951-957
关键词	jolkinolide B neuroendocrine differentiation prostate cancer cell lines growth arrest apoptosis
ISSN号	0006-2952
DOI	10.1016/S0006-2952(01)00938-8
文献子类	Article
英文摘要	Euphorbia fischeriana is a Chinese herbal medicine which has been reported to possess chemotherapeutic effects, yet the underlying mechanism is unclear. In order to understand its possible anti-tumor property, we have isolated a number of chemical compounds from the roots of this plant [Phytochemistry 52 (1999) 117] and studied their in vitro effects by using human prostate LNCaP cancer cell line. Among the six compounds tested, jolkinolide B exhibited the most potent anti-proliferative activity (IC50 = 12.5 mug/mL = 40 muM) and it inhibited DNA synthesis by down-regulating bromodeoxyuridine (BrdU) incorporation in LNCaP cells in a dose-dependent manner. Jolkinolide B, at concentrations up to 25 mug/mL, induced G 1 arrest and neuroendocrine differentiation of LNCaP cells. Immunoblotting analysis confirmed the increased expression of neuroendocrine markers, keratin 8/18 (K8/18) and neuron specific enolase (NSE), in these cells. Apoptotic bodies and DNA fragmentation were observed by fluorescence microscopy and flow cytometry when the cells were exposed to a concentration higher than 25 mug/mL jolkinolide B. Taken all data together, jolkinolide B seems to play a role in the regulation of proliferation, differentiation, and apoptosis of LNCaP cells. (C) 2002 Elsevier Science Inc. All rights reserved.
WOS关键词	EUPHORBIA-FISCHERIANA ; CARCINOMA-CELLS ; APOPTOSIS ; GROWTH ; ACID ; PROLIFERATION ; INDUCTION ; GLAND ; AMP
WOS研究方向	Pharmacology & Pharmacy
语种	英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD
WOS记录号	WOS:000175113600016
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/274377]
专题	中国科学院上海药物研究所
通讯作者	Liu, WK
作者单位	1.Chinese Univ Hong Kong, Sch Chinese Med, Shatin, Hong Kong, Peoples R China 2.Chinese Univ Hong Kong, Fac Med, Dept Anat, Shatin, Hong Kong, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Liu, WK,Ho, JCK,Qin, GW,et al. Jolkinolide B induces neuroendocrine differentiation of human prostate LNCaP cancer cell line[J]. BIOCHEMICAL PHARMACOLOGY,2002,63(5):951-957.
APA	Liu, WK,Ho, JCK,Qin, GW,&Che, CT.(2002).Jolkinolide B induces neuroendocrine differentiation of human prostate LNCaP cancer cell line.BIOCHEMICAL PHARMACOLOGY,63(5),951-957.
MLA	Liu, WK,et al."Jolkinolide B induces neuroendocrine differentiation of human prostate LNCaP cancer cell line".BIOCHEMICAL PHARMACOLOGY 63.5(2002):951-957.