Structural characterization of the fusion core in syncytin, envelope protein of human endogenous retrovirus family W | |
Gong, R; Peng, XX; Kang, SL; Feng, HX; Huang, JY; Zhang, WT; Lin, DH; Tien, P; Xiao, GF | |
刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS |
2005-06-17 | |
卷号 | 331期号:4页码:1193-1200 |
关键词 | syncytin fusion human endogenous retrovirus envelope protein heptad repeats trophoblast cell |
ISSN号 | 0006-291X |
DOI | 10.1016/j.bbrc.2005.04.032 |
文献子类 | Article |
英文摘要 | Syncytin is a captive retroviral envelope protein, possibly involved in the formation of the placental syncytiotrophoblast layer generated by trophoblast cell fusion at the maternal-fetal interface. We found that syncytin and type I viral envelope proteins shared similar structural profiling, especially in the regions of N- and C-terminal heptad repeats (NHR and CHR). We expressed the predicted regions of NHR (41 aa) and CHR (34 aa) in syncytin as a native single chain (named 2-helix protein) to characterize it. 2-helix protein exists as a trimer and is highly a-helix, thermo-stable, and denatured by low pH. NHR and CHR could form a protease-resistant complex. The complex structure built by the molecular docking demonstrated that NHR and CHR associated in an antiparallel manner. Overall, the 2-helix protein could form a thermo-stable coiled coil trimer. The fusion core structure of syncytin was first demonstrated in endogenous retrovirus. These results support the explanation how syncytin mediates cytotrophoblast cell fusion involved in placental morphogenesis. (c) 2005 Elsevier Inc. All rights reserved. |
WOS关键词 | CIRCULAR-DICHROISM SPECTRA ; HEPTAD REPEAT REGIONS ; HERV-W ; FUNCTIONAL-CHARACTERIZATION ; INFLUENZA HEMAGGLUTININ ; SECONDARY STRUCTURE ; 6-HELIX BUNDLE ; SPIKE PROTEIN ; CELL-FUSION ; VIRUS |
WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
语种 | 英语 |
出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
WOS记录号 | WOS:000229234500042 |
内容类型 | 期刊论文 |
源URL | [http://119.78.100.183/handle/2S10ELR8/273852] |
专题 | 中国科学院上海药物研究所 |
通讯作者 | Tien, P |
作者单位 | 1.Wuhan Univ, Coll Life Sci, State Key Lab Virol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Meteria Med, Shanghai 201203, Peoples R China |
推荐引用方式 GB/T 7714 | Gong, R,Peng, XX,Kang, SL,et al. Structural characterization of the fusion core in syncytin, envelope protein of human endogenous retrovirus family W[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2005,331(4):1193-1200. |
APA | Gong, R.,Peng, XX.,Kang, SL.,Feng, HX.,Huang, JY.,...&Xiao, GF.(2005).Structural characterization of the fusion core in syncytin, envelope protein of human endogenous retrovirus family W.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,331(4),1193-1200. |
MLA | Gong, R,et al."Structural characterization of the fusion core in syncytin, envelope protein of human endogenous retrovirus family W".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 331.4(2005):1193-1200. |
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