Triptolide inhibits COX-2 expression via NF-kappa B pathway in astrocytes

doi:10.1016/j.neures.2006.02.013

	Triptolide inhibits COX-2 expression via NF-kappa B pathway in astrocytes
	Dai, Yu-Qiao ; Jin, Dao-Zhong ; Zhu, Xing-Zu ; Lei, De-Liang
刊名	NEUROSCIENCE RESEARCH
	2006-06
卷号	55 期号:2 页码:154-160
关键词	triptolide cyclooxygenase-2 lipopolysaccharide NF-kappa B
ISSN号	0168-0102
DOI	10.1016/j.neures.2006.02.013
文献子类	Article
英文摘要	Previous investigations have showed that triptolide possessed potent anti-inflammatory and immunosuppressive properties. In the present study, we examined the protective effects of triptolide on the inflammatory response induced by bacterial lipopolysaccharide (LPS) both in vivo and in vitro. Intrahippocampal injection of LPS (4 mu g) in rats significantly increased the immunoreactivity of glial fibrillary acid protein (GFAP) and cyclooxygenase-2 (COX-2) in the injected region, which was reduced by pretreatment with triptolide (10-50 mu g/kg) for 5 d. In the cultured human differentiated A172 astroglial cells, LPS (1 mg/L) increased the expression of COX-2 mRNA and protein, the production of prostaglandin E-2 (PGE(2)) and the DNA binding activity of NF-kappa B, which were markedly attenuated by pretreatment with triptolide (0.2-5 mu g/L) for I h. These results suggested that the protective effect of triptolide on neuroinflammation is mediated by decreasing COX-2 expression, at least partly, via the inhibition of NF-kappa B signaling pathway. (c) 2006 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.
WOS关键词	NITRIC-OXIDE SYNTHASE ; TUMOR-NECROSIS-FACTOR ; CYCLOOXYGENASE-2 COX-2 ; TRIPTERYGIUM-WILFORDII ; DOPAMINERGIC-NEURONS ; PROSTAGLANDIN E-2 ; ENZYME-ACTIVITY ; BRAIN-INJURY ; TNF-ALPHA ; CELLS
WOS研究方向	Neurosciences & Neurology
语种	英语
出版者	ELSEVIER IRELAND LTD
WOS记录号	WOS:000238012400007
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/273581]
专题	中国科学院上海药物研究所
通讯作者	Lei, De-Liang
作者单位	1.Cent S Univ, Dept Anat & Neurobiol, Xiang Ya Sch Med, Changsha 410013, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Dai, Yu-Qiao,Jin, Dao-Zhong,Zhu, Xing-Zu,et al. Triptolide inhibits COX-2 expression via NF-kappa B pathway in astrocytes[J]. NEUROSCIENCE RESEARCH,2006,55(2):154-160.
APA	Dai, Yu-Qiao,Jin, Dao-Zhong,Zhu, Xing-Zu,&Lei, De-Liang.(2006).Triptolide inhibits COX-2 expression via NF-kappa B pathway in astrocytes.NEUROSCIENCE RESEARCH,55(2),154-160.
MLA	Dai, Yu-Qiao,et al."Triptolide inhibits COX-2 expression via NF-kappa B pathway in astrocytes".NEUROSCIENCE RESEARCH 55.2(2006):154-160.