Antimicrobial activities of YycG histidine kinase inhibitors against Staphylococcus epidermidis biofilms

doi:10.1111/j.1574-6968.2007.00749.x

	Antimicrobial activities of YycG histidine kinase inhibitors against Staphylococcus epidermidis biofilms
	Qin, Zhiqiang ; Lee, Baoleri ; Yang, Lei ; Zhang, Jian ; Yang, Xiaomei ; Qu, Di ; Jiang, Hualiang; Molin, Soeren
刊名	FEMS MICROBIOLOGY LETTERS
	2007-08
卷号	273 期号:2 页码:149-156
关键词	biofilms nosocomial infections antibiotics resistance vancomycin
ISSN号	0378-1097
DOI	10.1111/j.1574-6968.2007.00749.x
文献子类	Article
英文摘要	Staphylococcus epidermidis has become a significant pathogen causing infections due to biofilm formation on surfaces of indwelling medical devices. Biofilm-associated bacteria exhibit enhanced resistance to many conventional antibiotics. It is therefore, important to design novel antimicrobial reagents targeting S. epidermidis biofilms. In a static chamber system, the bactericidal effect of two leading compounds active as YycG inhibitors was assessed on biofilm cells by confocal laser scanning microscopy combined with viability staining. In young biofilms (6-h-old), the two compounds killed the majority ofthe embedded cells at concentrations of 100 mu M and 25 mu M, respectively. In mature biofilms (24-h-old), one compound was still effectively killing biofilm cells, whereas the other compound mainly killed cells located at the bottom of the biofilm. In contrast, vancomycin was found to stimulate biofilm development at the M13C (8 mu g mL(-1)). Even at a high concentration (128 mu g mL(-1)), vancomycin exhibited poor killing on cells embedded in biofilms. The two compounds exhibited faster and more effective killing of S. epidermidis planktonic cells than vancomycin at the early stage of exposure (6 h). The data suggest that the new inhibitors can serve as potential agents against S. epidermidis biofilms when added alone or in concert with other antimicrobial agents.
WOS关键词	COAGULASE-NEGATIVE STAPHYLOCOCCI ; INDWELLING VASCULAR CATHETERS ; BACTERIAL BIOFILM ; IN-VITRO ; INFECTIONS ; EXPRESSION ; ANTIBIOTICS ; PERITONITIS ; TOBRAMYCIN ; RELEASE
WOS研究方向	Microbiology
语种	英语
出版者	BLACKWELL PUBLISHING
WOS记录号	WOS:000248800600004
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/273188]
专题	中国科学院上海药物研究所
通讯作者	Qu, Di
作者单位	1.Fudan Univ, Shanghai Med Sch, Inst Biomed Sci, Minist Educ & Publ Hlth,Key Lab Med Mol Virol, Shanghai 200032, Peoples R China 2.Fudan Univ, Shanghai Med Sch, Inst Med Microbiol, Shanghai 200032, Peoples R China 3.Tech Univ Denmark, Bioctr, Infect Microbiol Grp, DK-2800 Lyngby, Denmark 4.Chinese Acad Sci, Shanghai Inst Mat Med, Grad Sch, Drug Discovery & Design Ctr,State Key Lab Drug Re, Shanghai 200031, Peoples R China
推荐引用方式 GB/T 7714	Qin, Zhiqiang,Lee, Baoleri,Yang, Lei,et al. Antimicrobial activities of YycG histidine kinase inhibitors against Staphylococcus epidermidis biofilms[J]. FEMS MICROBIOLOGY LETTERS,2007,273(2):149-156.
APA	Qin, Zhiqiang.,Lee, Baoleri.,Yang, Lei.,Zhang, Jian.,Yang, Xiaomei.,...&Molin, Soeren.(2007).Antimicrobial activities of YycG histidine kinase inhibitors against Staphylococcus epidermidis biofilms.FEMS MICROBIOLOGY LETTERS,273(2),149-156.
MLA	Qin, Zhiqiang,et al."Antimicrobial activities of YycG histidine kinase inhibitors against Staphylococcus epidermidis biofilms".FEMS MICROBIOLOGY LETTERS 273.2(2007):149-156.