HPLC-fluorescent spectrometric determination of serum mexiletine concentration after derivatization with fluram

	HPLC-fluorescent spectrometric determination of serum mexiletine concentration after derivatization with fluram
	Zhang Hui 2; Yu Chen 2; Liu Gangyi 2; Jia Jingying 2; Hong Youcai 2; Xu Xiurong 1
刊名	Acta Pharmaceutica Sinica
	2003
卷号	38 期号:3 页码:215-217
关键词	mexiletine hydrochloride pre-column derivatization HPLC-FLU serum drug concentration
ISSN号	0513-4870
其他题名	血清中美西律的柱前衍生化－液相色谱荧光检测
文献子类	Article
英文摘要	Aim To establish an HPLC-fluorescent spectrometric method for the determination of mexiletine hydrochloride in plasma after derivatization with fluram. Methods Fluram acetone solution was added to the deproteinized plasma with acetone to obtain the derivative of mexiletine. The HPLC method was performed on a column of Allitima C-18 (150 mm * 4.6 mm, 5 ^m) with the mobile phase of methanol-water-diethylamine-phosphoric acid buffer (2.4 molL~ , pH 4.0) (7028*2) , and the detective wavelength were set at Ex 392 nm and Em 480 nm. Results Mexiletine has a liner range over the concentration range from 0.100-6.400 mg·L" . The lowest detectable concentration of this method was 5 jug·L~ (S//V^4) . The intra-day and inter-day RSDs were 1.34% -5.31%, respectively. Conclusion This method is simple, selective and can be used for therapeutic drug monitoring (TDM) and pharmacokinetic studies of mexiletine.
资助项目	上海市徐汇区卫生局科研基金资助项目(C01-55)[00000000]
WOS研究方向	Pharmacology & Pharmacy (provided by Clarivate Analytics)
语种	中文
CSCD记录号	CSCD:1334347
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/268468]
专题	中国科学院上海药物研究所
作者单位	1.Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 200031, China. 2.Shanghai Xu Hui District Central Hospital, Shanghai 200031, China.;
推荐引用方式 GB/T 7714	Zhang Hui,Yu Chen,Liu Gangyi,et al. HPLC-fluorescent spectrometric determination of serum mexiletine concentration after derivatization with fluram[J]. Acta Pharmaceutica Sinica,2003,38(3):215-217.
APA	Zhang Hui,Yu Chen,Liu Gangyi,Jia Jingying,Hong Youcai,&Xu Xiurong.(2003).HPLC-fluorescent spectrometric determination of serum mexiletine concentration after derivatization with fluram.Acta Pharmaceutica Sinica,38(3),215-217.
MLA	Zhang Hui,et al."HPLC-fluorescent spectrometric determination of serum mexiletine concentration after derivatization with fluram".Acta Pharmaceutica Sinica 38.3(2003):215-217.