Effects of ketamine on glutamate-induced apoptosis in neuronal PC 12 cells | |
Wang Li; Jiang Wei; Zhu Xinzu | |
刊名 | Chinese Journal of Anesthesiology
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2004 | |
卷号 | 24期号:6页码:450-452 |
关键词 | Ketamine PC12 cells Neurons Glutamic acid Apoptosis |
ISSN号 | 0254-1416 |
其他题名 | 氯胺酮对谷氨酸引起神经元样嗜铬细胞瘤细胞株凋亡的影响 |
文献子类 | Article |
英文摘要 | Objective To study the effects of ketamine on glutamate-induced apoptosis in neuronal cells using PC 12 pheochromocytoma cell line (provided by Chinese Academy of Phamacological Research) . Methods After being incubated in the culture medium containing 7S-NGF for 6 days. Over 95 % of the PC cells differentiated into neuron-type cells. The 7S-NGF induced dififerentiated neuronal PC 12 cells were seeded in 24-well plates pre-coated with poly-L-lysine(2 x 10s cells per well) .24 hours later the PC12 cells were exposed to glutamate 20 mmol/ L(group A); glutamate 20 mmol/L + ketamine 0.1 mmo/L (group B); glutamate 20 mmol/L + ketamine 1.0 mmol/L (group C) jglutamate 20 mmol/L + D-APS 100 funol/L(group D) and new culture medium containing no 7S-NGF(group E, control group) , and incubated for 18 hours .The viability of the cells was evaluated by the ability of the cells to reduce the tetragotium derivative MTT into a blue formagan salt. DNA fragmentation indicative of apoptosis was detected using TUNEL technique. Results in group A following incubation with glutamate 20 mmol/L for 18 h , at 37 V,, the viability was PC 12 cells was reduced to 37 % 6 % . However ketamine , when added to the culture medium to gather with glutamate , inhibited glutamate-induced cell death . The viability of PC12cell was 65 7% in group B an 99 10% in group C. Ketamine appeared to attenuate the apoptotic process, because the number of the apoptotic cell bodies, determinated by YUNEL was also reduced by ketamine, with only 15 ~ 20% of neuronal cells staining positive after exposure to 20 mmol/L glutamate .The difference between group A and C was very significant ( P < 0.01) .In addition D-APS 100 fimol /L ( a competitive antagonist) also exerted significant protective effects on neuronal PC 12 cells. Conclusion Ketamine protects neuronal PC 12 cells from glutamate neurotoxicity induced apoptosis. |
WOS研究方向 | Surgery (provided by Clarivate Analytics) |
语种 | 中文 |
CSCD记录号 | CSCD:1661276 |
内容类型 | 期刊论文 |
源URL | [http://119.78.100.183/handle/2S10ELR8/268365] ![]() |
专题 | 中国科学院上海药物研究所 |
作者单位 | Department of Anesthesiolagy, Sixth People's Hospital, Shanghai Jiaotong University, 200233. |
推荐引用方式 GB/T 7714 | Wang Li,Jiang Wei,Zhu Xinzu. Effects of ketamine on glutamate-induced apoptosis in neuronal PC 12 cells[J]. Chinese Journal of Anesthesiology,2004,24(6):450-452. |
APA | Wang Li,Jiang Wei,&Zhu Xinzu.(2004).Effects of ketamine on glutamate-induced apoptosis in neuronal PC 12 cells.Chinese Journal of Anesthesiology,24(6),450-452. |
MLA | Wang Li,et al."Effects of ketamine on glutamate-induced apoptosis in neuronal PC 12 cells".Chinese Journal of Anesthesiology 24.6(2004):450-452. |
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