In vitro study of the effects of estrogen receptor beta expression on the biological behavior of a human breast cancer cell line

	In vitro study of the effects of estrogen receptor beta expression on the biological behavior of a human breast cancer cell line
	Hou Yifeng; Yuan Shengtao; Li Hecheng; Wu Jiong; Lu Jinsong; Lu Lijuan; Liu Gang; Shen Zhenzhou; Ding Jian; Shao Zhimin
刊名	Chinese Journal of Oncology
	2005
卷号	27 期号:7 页码:389-392
关键词	Ets-1 p21 Breast neoplasms ERbeta MMPs Ets-1 p21
ISSN号	0253-3766
其他题名	雌激素受体beta亚型对人乳腺癌细胞株生物学特性的影响
文献子类	Article
英文摘要	Objective To study effects of estrogen receptor \|3 (ER \|3) on the biological behavior of a human breast cancer cell line MDA-MB-435. Methods Human ER [3 cDNA was introduced into MDA-MB-435 cells by stable transfection. Effects of ER (3 expression on cell proliferation and invasion were investigated by MTT, flow cytometry and transwell techniques. Cyclin A, cyclin E, cyclin Dl, p21, MMPs, Ets-1, VEGF and b-FGF were detected by RT-PCR and/or Western blot or gelatin zymography. Results ER (3 was shown to be able to significandy increase the proliferation and invasion of MDA-MB-435 cells in an estradiol-independent manner. The S phase distribution of the cells with ER (3 overexpression was 46.8% , significantly higher than thai of wild type (29.9%) and mock transfected cells (27.6%) (P = 0.0l). In ER (3 transfected cells, the expression of p21 decreased by 33.3 % at mRNA level ( P = 0.03) and by 47.4% at protein level (P = 0.02) , respectively. The expression of MMP-9 increased by 91.3 % at mRNA level (P < 0.01) and its activity was up-regulated by 67.3 % (P = 0.02). Furthermore, the mRNA and protein levels of Ets-1 increased 62.2% (P = 0.01) and 51.0% (P = 0.0l), respectively. No significant difference was observed in the mRNA levels of cyclin A, cyclin E, cyclin Dl, MMP-1, MMP-2, MMP-7, VEGF and b-FGF among these cells. Conclusion ER (3 can enhance proliferation and invasion of breast cancer cells. Down-regulation of p21 and up-regulation of MMP-9 and Ets-1 may be involved in its mechanisms.
资助项目	上海市科技发展基金资助项目[00000000]
WOS研究方向	Oncology (provided by Clarivate Analytics)
语种	中文
CSCD记录号	CSCD:2094244
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/268285]
专题	中国科学院上海药物研究所
作者单位	Department of Breast Surgery, Cancer Hospital, Fudan University, Shanghai 200032, China.
推荐引用方式 GB/T 7714	Hou Yifeng,Yuan Shengtao,Li Hecheng,et al. In vitro study of the effects of estrogen receptor beta expression on the biological behavior of a human breast cancer cell line[J]. Chinese Journal of Oncology,2005,27(7):389-392.
APA	Hou Yifeng.,Yuan Shengtao.,Li Hecheng.,Wu Jiong.,Lu Jinsong.,...&Shao Zhimin.(2005).In vitro study of the effects of estrogen receptor beta expression on the biological behavior of a human breast cancer cell line.Chinese Journal of Oncology,27(7),389-392.
MLA	Hou Yifeng,et al."In vitro study of the effects of estrogen receptor beta expression on the biological behavior of a human breast cancer cell line".Chinese Journal of Oncology 27.7(2005):389-392.