Determination of ambroxol and clenbuterol in human plasma by LC-MS/MS method

	Determination of ambroxol and clenbuterol in human plasma by LC-MS/MS method
	Lin, Nan; Chen, Xiao-Yan; Song, Bo; Zhong, Da-Fang
	2007-03
卷号	42
期号	3
页码	308-13
英文摘要	Ambroxol and clenbuterol were extracted from human plasma samples by liquid-liquid extraction, ambroxol was separated on a Zorbax XDB-C18 column and detected by tandem mass spectrometry with an atmospheric pressure chemical ionization interface after oral administration of a compound preparation. Clenbuterol was separated on a Zorbax XDB-C8 column and detected by tandem mass spectrometry with an electrospray ionization interface. Diphenhydramine is used as the internal standard. The linear concentration ranges of the calibration curves for ambroxol and clenbuterol were 0.080 - 400 microg x L(-1) and 5.0 - 5 000 ng x L(-1), respectively. The lower limits of quantification were 0.080 microg x L(-1) for ambroxol and 5.0 ng x L(-1) for clenbuterol, individually. The inter-day and intra-day precision (RSD) across three validation run over the entire concentration range was below 7.5%, and the accuracy (RE) was within +/- 2.5% for both ambroxol and clenbuterol. The methods were used to determine the pharmacokinetic parameters of ambroxol and clenbuterol in human plasma after oral administration of a compound preparation containing 60 mg ambroxol hydrochloride and 40 microg clenbuterol hydrochloride. The method was proved to be highly sensitive, selective and suitable for the pharmacokinetic study of different compound preparations containing ambroxol and clenbuterol.
会议录	Yao xue xue bao = Acta pharmaceutica Sinica
文献子类	Article;Meeting Abstract
语种	中文
内容类型	会议论文
源URL	[http://119.78.100.183/handle/2S10ELR8/266889]
专题	中国科学院上海药物研究所
作者单位	Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China
推荐引用方式 GB/T 7714	Lin, Nan,Chen, Xiao-Yan,Song, Bo,et al. Determination of ambroxol and clenbuterol in human plasma by LC-MS/MS method[C]. 见:.