Measuring the molecular force of Burkitt's lymphoma patient cells using AFM

doi:10.1109/NANOMED.2010.5749829

	Measuring the molecular force of Burkitt's lymphoma patient cells using AFM
	Li, Mi 1,2,6; Liu, Lianqing 1; Xi, Ning 1,3; Wang, Yuechao 1; Dong, Zaili 1; Li, Guangyong 1,4; Xiao, Xiubin 5; Zhang, Weijing 5
	2010
DOI	10.1109/NANOMED.2010.5749829
页码	176-179
英文摘要	The treatment of Non-Hodgkin's lymphoma (NHL) was revolutionized by the approval of rituximab in 1997. Rituximab is a CD20-directed monoclonal antibody (mAb). Despite the great success of rituximab in the treatment of B-cell NHL, the urgent need is to enhance the efficacy due to the markedly variable patient responses. Hence elucidating the molecular mechanisms of rituximab's anti-cancer effect is of great significance. In the past decade the atomic force microscopy (AFM) has proven to be a powerful tool for characterizing the morphological properties and measuring the physiological interaction forces of single cells and single molecules under native conditions. In this work, the AFM single-molecule force spectroscopy(SMFS) was applied to quantitatively measure the CD20-rituximab binding force on Burkitt's lymphoma patient bone marrow cells. The experimental results will facilitate further investigation of the molecular mechanisms of rituximab's anticancer effect.
会议录	2010 IEEE International Conference on Nano/Molecular Medicine and Engineering, IEEE NANOMED 2010
会议录出版者	IEEE Computer Society
文献子类	Proceedings Paper
语种	英语
内容类型	会议论文
源URL	[http://119.78.100.183/handle/2S10ELR8/266859]
专题	中国科学院上海药物研究所
通讯作者	Liu, Lianqing
作者单位	1.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, 110016, China; 2.Graduate University, Chinese Academy of Sciences, Beijing, 100049, China; 3.Department of Electrical and Computer Engineering, Michigan State University, East Lansing, MI 48824, United States; 4.Department of Electrical and Computer Engineering, University of Pittsburgh, Pittsburgh, PA 15261, United States; 5.Department of Lymphoma, Military Medical Academy of Sciences, Beijing, 100071, China; 6.State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China
推荐引用方式 GB/T 7714	Li, Mi,Liu, Lianqing,Xi, Ning,et al. Measuring the molecular force of Burkitt's lymphoma patient cells using AFM[C]. 见:.