Sulfobetaine-Vinylimidazole Block Copolymers: A Robust Quantum Dot Surface Chemistry Expanding Bioimaging's Horizons

doi:10.1021/acsnano.5b05705

	Sulfobetaine-Vinylimidazole Block Copolymers: A Robust Quantum Dot Surface Chemistry Expanding Bioimaging's Horizons
	Tasso, Mariana 1; Giovanelli, Emerson 1; Zala, Diana 2; Bouccara, Sophie 1; Fragola, Alexandra 1; Hanafi, Mohamed 3; Lenkei, Zsolt 2; Pons, Thomas 1; Lequeux, Nicolas 1
刊名	ACS nano
	2015-11-24
卷号	9 期号:11 页码:11479-89
ISSN号	1936-086X
DOI	10.1021/acsnano.5b05705
文献子类	Article
英文摘要	Long-term inspection of biological phenomena requires probes of elevated intra- and extracellular stability and target biospecificity. The high fluorescence and photostability of quantum dot (QD) nanoparticles contributed to foster their promise as bioimaging tools that could overcome limitations associated with traditional fluorophores. However, QDs' potential as a bioimaging platform relies upon a precise control over the surface chemistry modifications of these nano-objects. Here, a zwitterion-vinylimidazole block copolymer ligand was synthesized, which regroups all anchoring groups in one compact terminal block, while the rest of the chain is endowed with antifouling and bioconjugation moieties. By further application of an oriented bioconjugation approach with whole IgG antibodies, QD nanobioconjugates were obtained that display outstanding intra- and extracellular stability as well as biorecognition capacity. Imaging the internalization and intracellular dynamics of a transmembrane cell receptor, the CB1 brain cannabinoid receptor, both in HEK293 cells and in neurons, illustrates the breadth of potential applications of these nanoprobes.
语种	英语
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/266761]
专题	中国科学院上海药物研究所
作者单位	1.LPEM, ESPCI ParisTech, PSL Research University , CNRS UMR 8213, Sorbonne Universités, UPMC Univ. Paris 6, 10 rue Vauquelin, 75005 Paris, France; 2.Brain Plasticity Unit, ESPCI ParisTech, PSL Research University , CNRS UMR 8249, 10 rue Vauquelin, 75005 Paris, France; 3.SIMM, ESPCI ParisTech, PSL Research University , CNRS UMR 7615, Sorbonne Universités, UPMC Univ. Paris 6, 10 rue Vauquelin, 75005 Paris, France
推荐引用方式 GB/T 7714	Tasso, Mariana,Giovanelli, Emerson,Zala, Diana,et al. Sulfobetaine-Vinylimidazole Block Copolymers: A Robust Quantum Dot Surface Chemistry Expanding Bioimaging's Horizons[J]. ACS nano,2015,9(11):11479-89.
APA	Tasso, Mariana.,Giovanelli, Emerson.,Zala, Diana.,Bouccara, Sophie.,Fragola, Alexandra.,...&Lequeux, Nicolas.(2015).Sulfobetaine-Vinylimidazole Block Copolymers: A Robust Quantum Dot Surface Chemistry Expanding Bioimaging's Horizons.ACS nano,9(11),11479-89.
MLA	Tasso, Mariana,et al."Sulfobetaine-Vinylimidazole Block Copolymers: A Robust Quantum Dot Surface Chemistry Expanding Bioimaging's Horizons".ACS nano 9.11(2015):11479-89.