Analysis of chiral non-steroidal anti-inflammatory drugs flurbiprofen, ketoprofen and etodolac binding with HSA

doi:10.1016/j.jpha.2011.06.005

	Analysis of chiral non-steroidal anti-inflammatory drugs flurbiprofen, ketoprofen and etodolac binding with HSA
	Guo, Chang-Chuan; Tang, Yi-Hong; Hu, Hai-Hong; Yu, Lu-Shan; Jiang, Hui-Di; Zeng, Su
刊名	Journal of pharmaceutical analysis
	2011-08
卷号	1 期号:3 页码:184-190
ISSN号	2095-1779
DOI	10.1016/j.jpha.2011.06.005
文献子类	Article
英文摘要	The protein binding of non-steroidal anti-inflammatory drugs flurbiprofen, ketoprofen and etodolac with human serum albumin (HSA) was investigated using indirect chiral high performance liquid chromatography (HPLC) and ultrafiltration techniques. -(-)-1-(1-naphthyl)-ethylamine (-NEA) was utilized as chiral derivatization reagent and pre-column derivatization RP-HPLC method was established for the separation and assay of the three pairs of enantiomer. The method had good linear relationship over the investigated concentration range without interference. The average extraction efficiency was higher than 85% in different systems, and the intra-day and inter-day precisions were less than 15%. In serum albumin, the protein binding of etodolac enantiomers showed significant stereoselectivity that the affinity of -enantiomer was stronger than -enantiomer, and the stereoselectivity ratio reached 6.06; Flurbiprofen had only weak stereoselectivity in HSA, and ketoprofen had no stereoselectivity at all. Scatchard curves showed that all the three chiral drugs had two types of binding sites in HSA.
语种	英语
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/266738]
专题	中国科学院上海药物研究所
作者单位	Department of Pharmaceutical Analysis and Drug Metabolism, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China
推荐引用方式 GB/T 7714	Guo, Chang-Chuan,Tang, Yi-Hong,Hu, Hai-Hong,et al. Analysis of chiral non-steroidal anti-inflammatory drugs flurbiprofen, ketoprofen and etodolac binding with HSA[J]. Journal of pharmaceutical analysis,2011,1(3):184-190.
APA	Guo, Chang-Chuan,Tang, Yi-Hong,Hu, Hai-Hong,Yu, Lu-Shan,Jiang, Hui-Di,&Zeng, Su.(2011).Analysis of chiral non-steroidal anti-inflammatory drugs flurbiprofen, ketoprofen and etodolac binding with HSA.Journal of pharmaceutical analysis,1(3),184-190.
MLA	Guo, Chang-Chuan,et al."Analysis of chiral non-steroidal anti-inflammatory drugs flurbiprofen, ketoprofen and etodolac binding with HSA".Journal of pharmaceutical analysis 1.3(2011):184-190.