An LC-MS method for simultaneous determination of nine ginsenosides in rat plasma and its application in pharmacokinetic study

doi:10.1002/bmc.1508

	An LC-MS method for simultaneous determination of nine ginsenosides in rat plasma and its application in pharmacokinetic study
	Zhou, Dandan; Tong, Ling; Wan, Meixu; Wang, Gangli; Ye, Zhengliang; Wang, Zhengtao; Lin, Ruichao
刊名	Biomedical chromatography : BMC
	2011-06
卷号	25 期号:6 页码:720-6
ISSN号	1099-0801
DOI	10.1002/bmc.1508
文献子类	Article
英文摘要	A sensitive and reliable LC-ESI-MS method for simultaneous determination of nine ginsenosides (Rh(1), Rg(2), Rg(1), Rf, Re, Rd, Rc, Rb(2) and Rb(1)) in rat plasma was developed and validated using saikosaponin A as an internal standard. The samples were extracted by solid-phase extraction. Chromatographic separation was carried out on a Hypersil Gold C(18) column (100 × 2.1 mm, 5 µm) by stepwise gradient elution with water (0.1% formic acid, v/v) and acetonitrile as the mobile phase. Detection was determined by selective ion monitoring mode using electrospray ionization in the negative ion mode. Good linearity over the investigated concentration ranges was observed with the values of r higher than 0.9900. The intra- and inter-day precisions were all no more than 15% and the average recoveries varied from 71.8 to 91.7%. This quantitative measurement was successfully applied to a pharmacokinetic study of Yi-Qi-Fu-Mai injection.
语种	英语
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/266539]
专题	中国科学院上海药物研究所
作者单位	Key Laboratory of Standardization of Chinese Medicines of Ministry of Education, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, People's Republic of China
推荐引用方式 GB/T 7714	Zhou, Dandan,Tong, Ling,Wan, Meixu,et al. An LC-MS method for simultaneous determination of nine ginsenosides in rat plasma and its application in pharmacokinetic study[J]. Biomedical chromatography : BMC,2011,25(6):720-6.
APA	Zhou, Dandan.,Tong, Ling.,Wan, Meixu.,Wang, Gangli.,Ye, Zhengliang.,...&Lin, Ruichao.(2011).An LC-MS method for simultaneous determination of nine ginsenosides in rat plasma and its application in pharmacokinetic study.Biomedical chromatography : BMC,25(6),720-6.
MLA	Zhou, Dandan,et al."An LC-MS method for simultaneous determination of nine ginsenosides in rat plasma and its application in pharmacokinetic study".Biomedical chromatography : BMC 25.6(2011):720-6.