Rapidly directional biotransformation of tauroursodeoxycholic acid through engineered Escherichia coli

doi:10.1007/s10295-017-1935-y

	Rapidly directional biotransformation of tauroursodeoxycholic acid through engineered Escherichia coli
	Shi, Jie; Wang, Jie; Yu, Lu; Yang, Li; Zhao, Shujuan; Wang, Zhengtao
刊名	Journal of industrial microbiology & biotechnology
	2017-07
卷号	44 期号:7 页码:1073-1082
ISSN号	1476-5535
DOI	10.1007/s10295-017-1935-y
文献子类	Article
英文摘要	Bear bile powder is a precious medicinal material. It is characterized by high content of tauroursodeoxycholic acid (TUDCA) at a ratio of 1.0-1.5 to taurochenodeoxycholic acid (TCDCA). Here, we reported the biotransformation of tauroursodeoxycholic acid (TUDCA) through Escherichia coli engineered with a two-step mimic biosynthetic pathway of TUDCA from taurochenodeoxycholic acid (TCDCA). Two 7α-hydroxysteroid dehydrogenase (7α-HSDH) and two 7β-hydroxysteroid dehydrogenase (7β-HSDH) genes (named as α, α, β, and β) were selected and synthesized to create four pathway variants using ePathBrick. All could convert TCDCA to TUDCA and the one harboring α and β (pαβ) showed the strongest capability. Utilizing the oxidative and reductive properties of 7α- and 7β-HSDH, an ideal balance between TUDCA and TCDCA was established by optimizing the fermentation conditions. By applying the optimal condition, E. coli containing pαβ (BL-pαβ) produced up to 1.61 ± 0.13 g/L of TUDCA from 3.23 g/L of TCDCA at a ratio of 1.3 to TCDCA. This study provides a potential approach for bear bile substitute production from cheap and readily available chicken bile.
语种	英语
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/266535]
专题	中国科学院上海药物研究所
通讯作者	Zhao, Shujuan; Wang, Zhengtao
作者单位	The SATCM Key Laboratory for New Resources & Quality Evaluation of Chinese Medicine, The MOE Key Laboratory for Standardization of Chinese Medicines and Shanghai Key Laboratory of Complex Prescription, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China
推荐引用方式 GB/T 7714	Shi, Jie,Wang, Jie,Yu, Lu,et al. Rapidly directional biotransformation of tauroursodeoxycholic acid through engineered Escherichia coli[J]. Journal of industrial microbiology & biotechnology,2017,44(7):1073-1082.
APA	Shi, Jie,Wang, Jie,Yu, Lu,Yang, Li,Zhao, Shujuan,&Wang, Zhengtao.(2017).Rapidly directional biotransformation of tauroursodeoxycholic acid through engineered Escherichia coli.Journal of industrial microbiology & biotechnology,44(7),1073-1082.
MLA	Shi, Jie,et al."Rapidly directional biotransformation of tauroursodeoxycholic acid through engineered Escherichia coli".Journal of industrial microbiology & biotechnology 44.7(2017):1073-1082.