Curcumol from Rhizoma Curcumae suppresses epileptic seizure by facilitation of GABA(A) receptors

doi:10.1016/j.neuropharm.2014.02.009

	Curcumol from Rhizoma Curcumae suppresses epileptic seizure by facilitation of GABA(A) receptors
	Ding, Jing 2,3,4; Wang, Jing-Jing 4; Huang, Chen 4; Wang, Li 4; Deng, Shining 1; Xu, Tian-Le 4; Ge, Wei-Hong 2; Li, Wei-Guang 1,4; Li, Fei 1
刊名	Neuropharmacology
	2014-06
卷号	81 页码:244-55
ISSN号	1873-7064
DOI	10.1016/j.neuropharm.2014.02.009
文献子类	Article
英文摘要	Rhizoma Curcumae is a common Chinese dietary spice used in South Asia and China for thousands of years. As the main extract, Rhizoma Curcumae oil has attracted a great interest due to its newly raised therapeutic activities including its pharmacological effects upon central nervous system such as neuroprotection, cognitive enhancement, and anti-seizure efficacy; however the molecular mechanisms and the target identification remain to be established. Here we characterize an inhibitory effect of curcumol, a major bioactive component of Rhizoma Curcumae oil, on the excitability of hippocampal neurons in culture, the basal locomotor activity of freely moving animals, and the chemically induced seizure activity in vivo. Electrophysiological recording showed that acute application of curcumol significantly facilitated the γ-aminobutyric acid (GABA)-activated current in cultured mouse hippocampal neurons and in human embryonic kidney cells expressing α1- or α5-containing A type GABA (GABAA) receptors in a concentration-dependent manner. Measurement of tonic and miniature inhibitory postsynaptic GABAergic currents in hippocampal slices indicated that curcumol enhanced both forms of inhibition. In both pentylenetetrazole and kainate seizure models, curcumol suppressed epileptic activity in mice by prolonging the latency to clonic and tonic seizures and reducing the mortality as well as the susceptibility to seizure, presumably by facilitating the activation of GABAA receptors. Taken together, our results identified curcumol as a novel anti-seizure agent which inhibited neuronal excitability through enhancing GABAergic inhibition.
语种	英语
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/266494]
专题	中国科学院上海药物研究所
通讯作者	Li, Wei-Guang; Li, Fei
作者单位	1.Department of Developmental and Behavioral Pediatrics, Shanghai Institute of Pediatric Translational Medicine, Shanghai Children's Medical Center, Ministry of Education-Shanghai Key Laboratory of Children's Environmental Health, Shanghai Jiao Tong University School of Medicine, Shanghai 200129, China 2.Department of Chinese Materia Medica, College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou 310053, China; 3.Department of Developmental and Behavioral Pediatrics, Shanghai Institute of Pediatric Translational Medicine, Shanghai Children's Medical Center, Ministry of Education-Shanghai Key Laboratory of Children's Environmental Health, Shanghai Jiao Tong University School of Medicine, Shanghai 200129, China; 4.Departments of Anatomy and Embryology, Biochemistry and Molecular Cell Biology, Shanghai Key Laboratory for Tumor Microenvironment and Inflammation, Institute of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China;
推荐引用方式 GB/T 7714	Ding, Jing,Wang, Jing-Jing,Huang, Chen,et al. Curcumol from Rhizoma Curcumae suppresses epileptic seizure by facilitation of GABA(A) receptors[J]. Neuropharmacology,2014,81:244-55.
APA	Ding, Jing.,Wang, Jing-Jing.,Huang, Chen.,Wang, Li.,Deng, Shining.,...&Li, Fei.(2014).Curcumol from Rhizoma Curcumae suppresses epileptic seizure by facilitation of GABA(A) receptors.Neuropharmacology,81,244-55.
MLA	Ding, Jing,et al."Curcumol from Rhizoma Curcumae suppresses epileptic seizure by facilitation of GABA(A) receptors".Neuropharmacology 81(2014):244-55.