Protective effect of naringenin against experimental colitis via suppression of Toll-like receptor 4/NF-κB signalling

doi:10.1017/S0007114512005594

	Protective effect of naringenin against experimental colitis via suppression of Toll-like receptor 4/NF-κB signalling
	Dou, Wei; Zhang, Jingjing; Sun, Aning; Zhang, Eryun; Ding, Lili; Mukherjee, Subhajit; Wei, Xiaohui; Chou, Guixin; Wang, Zheng-Tao; Mani, Sridhar
刊名	The British journal of nutrition
	2013-08
卷号	110 期号:4 页码:599-608
ISSN号	1475-2662
DOI	10.1017/S0007114512005594
文献子类	Article
英文摘要	Naringenin, one of the most abundant flavonoids in citrus, grapefruits and tomatoes, has been used as a traditional anti-inflammatory agent for centuries. However, the molecular mechanism of naringenin in intestinal inflammation remains unknown so far. The present study investigated a molecular basis for the protective effect of naringenin in dextran sulphate sodium-induced murine colitis. Pre-administration of naringenin significantly reduced the severity of colitis and resulted in down-regulation of pro-inflammatory mediators (inducible NO synthase (iNOS), intercellular adhesion molecule-1 (ICAM-1), monocyte chemoattractant protein-1 (MCP-1), cyclo-oxygenase-2 (Cox2), TNF-α and IL-6 mRNA) in the colon mucosa. The decline in the production of pro-inflammatory cytokines, specifically TNF-α and IL-6, correlated with a decrease in mucosal Toll-like receptor 4 (TLR4) mRNA and protein. Phospho-NF-κB p65 protein was significantly decreased, which correlated with a similar decrease in phospho-IκBα protein. Consistent with the in vivo results, naringenin exposure blocked lipopolysaccharide-stimulated nuclear translocation of NF-κB p65 in mouse macrophage RAW264.7 cells. In addition, in vitro NF-κB reporter assays performed on human colonic HT-29 cells exposed to naringenin demonstrated a significant inhibition of TNF-α-induced NF-κB luciferase expression. Thus, for the first time, the present study indicates that targeted inhibition of the TLR4/NF-κB signalling pathway might be an important mechanism for naringenin in abrogating experimental colitis.
语种	英语
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/266492]
专题	中国科学院上海药物研究所
作者单位	Shanghai Key Laboratory of Formulated Chinese Medicines and MOE Key Laboratory for Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, People's Republic of China
推荐引用方式 GB/T 7714	Dou, Wei,Zhang, Jingjing,Sun, Aning,et al. Protective effect of naringenin against experimental colitis via suppression of Toll-like receptor 4/NF-κB signalling[J]. The British journal of nutrition,2013,110(4):599-608.
APA	Dou, Wei.,Zhang, Jingjing.,Sun, Aning.,Zhang, Eryun.,Ding, Lili.,...&Mani, Sridhar.(2013).Protective effect of naringenin against experimental colitis via suppression of Toll-like receptor 4/NF-κB signalling.The British journal of nutrition,110(4),599-608.
MLA	Dou, Wei,et al."Protective effect of naringenin against experimental colitis via suppression of Toll-like receptor 4/NF-κB signalling".The British journal of nutrition 110.4(2013):599-608.