Identification of metabolites of adonifoline, a hepatotoxic pyrrolizidine alkaloid, by liquid chromatography/tandem and high-resolution mass spectrometry

doi:10.1002/rcm.4329

	Identification of metabolites of adonifoline, a hepatotoxic pyrrolizidine alkaloid, by liquid chromatography/tandem and high-resolution mass spectrometry
	Xiong, Aizhen; Yang, Li; He, Yuqi; Zhang, Fang; Wang, Jun; Han, Han; Wang, Changhong; Bligh, S W Annie; Wang, Zhengtao
刊名	Rapid communications in mass spectrometry : RCM
	2009-12
卷号	23 期号:24 页码:3907-16
ISSN号	1097-0231
DOI	10.1002/rcm.4329
文献子类	Article
英文摘要	Hepatotoxic pyrrolizidine alkaloid (HPA)-containing plants have always been a threat to human and livestock health worldwide. Adonifoline, a main HPA in Senecio scandens Buch.-Ham. ex D. Don (Qianli guang), was used officially as an infusion in cases of oral and pharyngeal infections in China. In this study in vivo metabolism of adonifoline was studied for the first time by identifying the metabolites of adonifoline present in bile, urine and feces of rats using liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS(n)) (ion trap) as well as liquid chromatography/electrospray ionization high-resolution mass spectrometry (LC/ESI-HRMS) (quadrupole-time of flight). In total 19 metabolites were identified and, among them, retronecine-N-oxides were confirmed by matching their fragmentation patterns with their fully characterized synthetic compounds. These metabolites are all involved in both phase I and phase II metabolic processes and the principal in vivo metabolism pathways of adonifoline were proposed.
语种	英语
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/266488]
专题	中国科学院上海药物研究所
作者单位	The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201210, China
推荐引用方式 GB/T 7714	Xiong, Aizhen,Yang, Li,He, Yuqi,et al. Identification of metabolites of adonifoline, a hepatotoxic pyrrolizidine alkaloid, by liquid chromatography/tandem and high-resolution mass spectrometry[J]. Rapid communications in mass spectrometry : RCM,2009,23(24):3907-16.
APA	Xiong, Aizhen.,Yang, Li.,He, Yuqi.,Zhang, Fang.,Wang, Jun.,...&Wang, Zhengtao.(2009).Identification of metabolites of adonifoline, a hepatotoxic pyrrolizidine alkaloid, by liquid chromatography/tandem and high-resolution mass spectrometry.Rapid communications in mass spectrometry : RCM,23(24),3907-16.
MLA	Xiong, Aizhen,et al."Identification of metabolites of adonifoline, a hepatotoxic pyrrolizidine alkaloid, by liquid chromatography/tandem and high-resolution mass spectrometry".Rapid communications in mass spectrometry : RCM 23.24(2009):3907-16.