Separation and identification of specific components and quality standard of stem of Dendrobium officinale

doi:10.4268/cjcmm20161318

	Separation and identification of specific components and quality standard of stem of Dendrobium officinale
	Ye, Zi; Lu, Ye; Xue, Ya-Fu; Xu, Hong; Wang, Zheng-Tao
刊名	Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica
	2016-07
卷号	41 期号:13 页码:2481-2486
ISSN号	1001-5302
DOI	10.4268/cjcmm20161318
文献子类	Article
英文摘要	The violanthin, a specific component, was separated and identified from the stems of Dendrobium officinale by chromatographic technique and spectroscopic method for the first time. The microscopic characteristics of D. officinale powder were examined under a microscopy and described. Thin layer chromatography (TLC) method was used for qualitative analysis of the violanthin from D. officinale stems with a mixture of ethyl acetate, butanone, formic acid and water (4∶3∶1∶1) as the developing solvent on high performance silica gel precoated plate (SGF254) and using aluminium trichloride as a chromagenic agent. The results showed significant characteristics of violanthin from D. officinale stems on TLC, with certain specificity, and could be used to distinguish it from other easily confusing processed medicinal stems of D. devonianum, D. gratiosissimum and D. aphyllum. The content of naringenin, an active ingredient in D. officinale stems was determined by HPLC analysis on a Bischoff Chromatography HIPAK NC-04 ODS AB column (4.4 mm×250 mm, 5 mm) with acetonitrile-0.1% phosphoric acid solution as the mobile phase for gradient elution. The wavelength was set at 226 nm and column temperature was 25 ℃. The HPLC method showed good linearity within the range of 3.90-250.00 g•mL⁻¹ (r = 0.999 9) for naringenin. The average recovery of naringenin was 99.20% with 0.17% of RSD. The mass fraction of 20 batches of D. officinale stems was between 0.190 and 0.498 mg•g⁻¹. The established qualitative and quantitative method was simple and rapid with good repeatability and accuracy, providing experimental basis for improving the quality standard of D. officinale, with a very important significance to ensure its quality and clinical effect.
语种	中文
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/266468]
专题	中国科学院上海药物研究所
作者单位	Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine(TCM), the Ministry of Education Key Laboratory for Standardization of Chinese Medicine, State Administration of TCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicine, Shanghai 201203, China
推荐引用方式 GB/T 7714	Ye, Zi,Lu, Ye,Xue, Ya-Fu,et al. Separation and identification of specific components and quality standard of stem of Dendrobium officinale[J]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica,2016,41(13):2481-2486.
APA	Ye, Zi,Lu, Ye,Xue, Ya-Fu,Xu, Hong,&Wang, Zheng-Tao.(2016).Separation and identification of specific components and quality standard of stem of Dendrobium officinale.Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica,41(13),2481-2486.
MLA	Ye, Zi,et al."Separation and identification of specific components and quality standard of stem of Dendrobium officinale".Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica 41.13(2016):2481-2486.