Absorption mechanism of neobavaisoflavone in Caco-2 cell monolayer mode

doi:10.4268/cjcmm20161528

	Absorption mechanism of neobavaisoflavone in Caco-2 cell monolayer mode
	Xu, Xiao-Kun; Chen, Zhi-Yong; Li, Zhuo-Qing; Zhang, Yi-Min; Liao, Li-Ping; Zhou, Qiang; Zhang, Zi-Jia
刊名	Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica
	2016-08
卷号	41 期号:15 页码:2922-2926
ISSN号	1001-5302
DOI	10.4268/cjcmm20161528
文献子类	Article
英文摘要	Neobavaisoflavone is one of flavonoids of traditional Chinese medicine Psoralea corylifolial. It has numerous biological properties such as antibacterial, anti-inflammatory, anti-cancer, and anti-osteoporosis effects. This paper aimed to investigate the absorption mechanism of neobavaisoflavone in Caco-2 cell monolayer model. The analyte and osalmide were separated on Thermo Syncronis C18 column with methanol-0.1% formic acid solution (90∶10) as the mobile phase, at a flow rate of 0.2 mL•min⁻¹. The concentration of neobavaisoflavone was determined in eletrospray ionization(ESI) positive ion mode with osalmide as an the internal standard. The effects of time, concentration, P-gp inhibitor verapamil, MRP-2 inhibitor MK-571 and BCRP inhibitor Ko143 on the absorption of neobavaisoflavone were investigated. According to the results, neobavaisoflavone showed a good linearity within the concentration of 10-2 000 μg•L⁻¹, and the results of its specificity, matrix effect, extraction recovery, precision, accuracy and stability all met the requirements. In the Caco-2 cell monolayer model, the transport volume of neobavaisoflavone was correlated positively with the time and concentration. The ER values of 15, 30, 50 μmol•L⁻¹ neobavaisoflavone were 1.64, 1.94,0.99, respectively. As compared with the control group, all of verapamil hyduochloride, MK-571 and Ko143 could promote the transportation of neobavaisoflavone, and the effect was more obvious in verapamil hyduochloride and Ko143. The absorption of neobavaisoflavone may be mainly of active transport in Caco-2 cell monolayer model, and also involve passive transport. Excretion mechanism of intestinal transport protein may be also involved.
语种	中文
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/266451]
专题	中国科学院上海药物研究所
作者单位	The MOE Key Laboratory for Standardization of Chinese Medicines, Shanghai Key Laboratory for Compound Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
推荐引用方式 GB/T 7714	Xu, Xiao-Kun,Chen, Zhi-Yong,Li, Zhuo-Qing,et al. Absorption mechanism of neobavaisoflavone in Caco-2 cell monolayer mode[J]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica,2016,41(15):2922-2926.
APA	Xu, Xiao-Kun.,Chen, Zhi-Yong.,Li, Zhuo-Qing.,Zhang, Yi-Min.,Liao, Li-Ping.,...&Zhang, Zi-Jia.(2016).Absorption mechanism of neobavaisoflavone in Caco-2 cell monolayer mode.Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica,41(15),2922-2926.
MLA	Xu, Xiao-Kun,et al."Absorption mechanism of neobavaisoflavone in Caco-2 cell monolayer mode".Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica 41.15(2016):2922-2926.