Intracellular glutathione plays important roles in pyrrolizidine alkaloids-induced growth inhibition on hepatocytes

doi:10.1016/j.etap.2009.06.002

	Intracellular glutathione plays important roles in pyrrolizidine alkaloids-induced growth inhibition on hepatocytes
	Chen, Ying 1,2; Ji, Lili ; Wang, Haitao ; Wang, Zhengtao
刊名	Environmental toxicology and pharmacology
	2009-11
卷号	28 期号:3 页码:357-62
ISSN号	1872-7077
DOI	10.1016/j.etap.2009.06.002
文献子类	Article
英文摘要	Pyrrolizidine alkaloids (PAs) are well-known natural hepatotoxins distributed widely in thousands of plants in the world. Adonifoline (Adon), senecionine (Sene) and monocrotaline (Mono) are retronecine-type PAs, and the present study is designed to observe the effects of intracellular glutathione on toxicity of these three PAs in human normal liver L-02 cells. The ratio of cellular reduced glutathione (GSH) and oxidized glutathione (GSSG) was assayed after L-02 cells were incubated with these three PAs for various times. Results showed that Adon, Sene and Mono all significantly decreased the ratio of GSH/GSSG in L-02 cells in the time- and concentration-dependent manner. The results of 3-(4,5-dimethylthiazol-2-yl) 2,5-diphenyltetrazolium bromide (MTT) and trypan blue staining assay showed that these three PAs all significantly decreased cell viability in L-02 cells when pretreated with 10μM BSO (L-Buthionine-S-R-Sulfoximine) for 24h to deplete intracellular GSH. Further results showed that anti-oxidant compounds such as NAC (N-Acetyl-Cysteine) and GSH could rescue the cytotoxicity caused by these three PAs with BSO pretreatment. Taken together, those results suggest that intracellular GSH plays important roles in regulating the cytotoxicity induced by PAs.
语种	英语
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/266443]
专题	中国科学院上海药物研究所
作者单位	1.Department of Pharmacognosy, China Pharmaceutical University, Nanjing, 210038, PR China; 2.Key Laboratory of Standardization of Chinese Medicines of Ministry of Education, Shanghai Key Laboratory of Complex Prescription, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, PR China
推荐引用方式 GB/T 7714	Chen, Ying,Ji, Lili,Wang, Haitao,et al. Intracellular glutathione plays important roles in pyrrolizidine alkaloids-induced growth inhibition on hepatocytes[J]. Environmental toxicology and pharmacology,2009,28(3):357-62.
APA	Chen, Ying,Ji, Lili,Wang, Haitao,&Wang, Zhengtao.(2009).Intracellular glutathione plays important roles in pyrrolizidine alkaloids-induced growth inhibition on hepatocytes.Environmental toxicology and pharmacology,28(3),357-62.
MLA	Chen, Ying,et al."Intracellular glutathione plays important roles in pyrrolizidine alkaloids-induced growth inhibition on hepatocytes".Environmental toxicology and pharmacology 28.3(2009):357-62.