Mangiferin attenuates the symptoms of dextran sulfate sodium-induced colitis in mice via NF-κB and MAPK signaling inactivation

doi:10.1016/j.intimp.2014.08.025

	Mangiferin attenuates the symptoms of dextran sulfate sodium-induced colitis in mice via NF-κB and MAPK signaling inactivation
	Dou, Wei 2; Zhang, Jingjing 2; Ren, Gaiyan 2; Ding, Lili 2; Sun, Aning 2; Deng, Chao 2; Wu, Xiaojun 2; Wei, Xiaohui 2; Mani, Sridhar 1; Wang, Zhengtao 2
刊名	International immunopharmacology
	2014-11
卷号	23 期号:1 页码:170-8
ISSN号	1878-1705
DOI	10.1016/j.intimp.2014.08.025
文献子类	Article
英文摘要	Inflammatory bowel disease (IBD) is a chronic and relapsing inflammatory disorder of the gastrointestinal (GI) tract, and currently no curative treatment is available. Mangiferin, a natural glucosylxanthone mainly from the fruit, leaves and stem bark of a mango tree, has a strong anti-inflammatory activity. We sought to investigate whether mangiferin attenuates inflammation in a mouse model of chemically induced IBD. Pre-administration of mangiferin significantly attenuated dextran sulfate sodium (DSS)-induced body weight loss, diarrhea, colon shortening and histological injury, which correlated with the decline in the activity of myeloperoxidase (MPO) and the level of tumor necrosis factor-α (TNF-α) in the colon. DSS-induced degradation of inhibitory κBα (IκBα) and the phosphorylation of nuclear factor-kappa B (NF-κB) p65 as well as the mRNA expression of pro-inflammatory mediators (inducible NO synthase (iNOS), intercellular adhesion molecule-1 (ICAM-1), TNF-α, interleukin-1β (IL-1β) and IL-6) in the colon were also downregulated by mangiferin treatment. Additionally, the phosphorylation/activation of DSS-induced mitogen-activated protein kinase (MAPK) proteins was also inhibited by mangiferin treatment. In accordance with the in vivo results, mangiferin exposure blocked TNF-α-stimulated nuclear translocation of NF-κB in RAW264.7 mouse macrophage cells. Transient transfection gene reporter assay performed in TNF-α-stimulated HT-29 human colorectal adenocarcinoma cells indicated that mangiferin inhibits NF-κB transcriptional activity in a dose-dependent manner. The current study clearly demonstrates a protective role for mangiferin in experimental IBD through NF-κB and MAPK signaling inhibition. Since mangiferin is a natural compound with little toxicity, the results may contribute to the effective utilization of mangiferin in the treatment of human IBD.
语种	英语
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/266421]
专题	中国科学院上海药物研究所
通讯作者	Wang, Zhengtao
作者单位	1.Department of Medicine, Albert Einstein College of Medicine, NY 10461, USA 2.Shanghai Key Laboratory of Formulated Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;
推荐引用方式 GB/T 7714	Dou, Wei,Zhang, Jingjing,Ren, Gaiyan,et al. Mangiferin attenuates the symptoms of dextran sulfate sodium-induced colitis in mice via NF-κB and MAPK signaling inactivation[J]. International immunopharmacology,2014,23(1):170-8.
APA	Dou, Wei.,Zhang, Jingjing.,Ren, Gaiyan.,Ding, Lili.,Sun, Aning.,...&Wang, Zhengtao.(2014).Mangiferin attenuates the symptoms of dextran sulfate sodium-induced colitis in mice via NF-κB and MAPK signaling inactivation.International immunopharmacology,23(1),170-8.
MLA	Dou, Wei,et al."Mangiferin attenuates the symptoms of dextran sulfate sodium-induced colitis in mice via NF-κB and MAPK signaling inactivation".International immunopharmacology 23.1(2014):170-8.