Identification of metabolites of geniposide in rat urine using ultra-performance liquid chromatography combined with electrospray ionization quadrupole time-of-flight tandem mass spectrometry

doi:10.1002/rcm.5216

	Identification of metabolites of geniposide in rat urine using ultra-performance liquid chromatography combined with electrospray ionization quadrupole time-of-flight tandem mass spectrometry
	Han, Han; Yang, Li; Xu, Ying; Ding, Yue; Bligh, S W Annie; Zhang, Tong; Wang, Zhengtao
刊名	Rapid communications in mass spectrometry : RCM
	2011-11-15
卷号	25 期号:21 页码:3339-50
ISSN号	1097-0231
DOI	10.1002/rcm.5216
文献子类	Article
英文摘要	Geniposide, an iridoid glycoside, is an important and characteristic compound in the fruits of Gardenia jasminoides Ellis, a commonly used medicinal herb in Chinese traditional and folk medicine for the treatment of inflammation and jaundice. However, few studies have been carried out on the metabolism of geniposide. In this study, we have established a rapid and sensitive method using ultra-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UPLC/ESI-QTOF-MS) for analysis of the metabolic profile of geniposide in rat urine after oral administration. A total of ten metabolites were detected and identified by comparing their fragmentation patterns with that of geniposide using Metabolynx™ and MassFragment™ software tools. The results revealed that the principal metabolism pathways of geniposide in rat occurred after deglycosylation of the irdoid glycoside take place and this is followed by glucuronidation and the pyran-ring cleavages. The major metabolite, the glucuronic acid conjugate of genipin as observed in vivo, was further confirmed by the in vitro enzymatic study. The results of this work have demonstrated the feasibility of the UPLC/ESI-QTOF-MS approach for rapid and reliable characterization of metabolites from iridoid compounds.
语种	英语
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/266412]
专题	中国科学院上海药物研究所
作者单位	The Ministry of Education (MOE) Key Laboratory for Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201210, China
推荐引用方式 GB/T 7714	Han, Han,Yang, Li,Xu, Ying,et al. Identification of metabolites of geniposide in rat urine using ultra-performance liquid chromatography combined with electrospray ionization quadrupole time-of-flight tandem mass spectrometry[J]. Rapid communications in mass spectrometry : RCM,2011,25(21):3339-50.
APA	Han, Han.,Yang, Li.,Xu, Ying.,Ding, Yue.,Bligh, S W Annie.,...&Wang, Zhengtao.(2011).Identification of metabolites of geniposide in rat urine using ultra-performance liquid chromatography combined with electrospray ionization quadrupole time-of-flight tandem mass spectrometry.Rapid communications in mass spectrometry : RCM,25(21),3339-50.
MLA	Han, Han,et al."Identification of metabolites of geniposide in rat urine using ultra-performance liquid chromatography combined with electrospray ionization quadrupole time-of-flight tandem mass spectrometry".Rapid communications in mass spectrometry : RCM 25.21(2011):3339-50.