Regulation of Vitreoscilla hemoglobin on biosynthesis of astragaloside IV

	Regulation of Vitreoscilla hemoglobin on biosynthesis of astragaloside IV
	Wang, Zi-Yan; Hu, Zhi-Bi; Wang, Zheng-Tao
刊名	Yao xue xue bao = Acta pharmaceutica Sinica
	2011-03
卷号	46 期号:3 页码:355-60
ISSN号	0513-4870
文献子类	Article
英文摘要	In the present study, the regulation of Vitreoscilla hemoglobin (VHb) on astragaloside IV biosynthesis was investigated. An intermediate expression vector consisting of the CaMV35S promoter fused to the vgb and nopaline synthase terminator was transferred into Astragalus membranaceus via Agrobacterium rhizogenes. The transgenic hairy roots were confirmed by PCR amplification and Southern blot hybridization. The expression of vgb in transgenic hairy roots was confirmed by RT-PCR. After 15 days cultivation, the dry weight and growth rate of transgenic hairy roots were higher than that of the non-transgenic hairy root. ELSD-HPLC analysis showed that astragaloside IV content of transgenic hairy roots was 5 to 6 times of non-transgenic hairy root control and 10 to 12 times of Radix Astragali from Shanxi Province. These results suggested that the expression of vgb promoted the growth of transgenic hairy roots, and increased the content of astragaloside IV.
语种	中文
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/266393]
专题	中国科学院上海药物研究所
作者单位	The SATCM Key Laboratory for New Resources & Quality Evaluation of Chinese Medicines and Shanghai Key Laboratory of Complex Prescription, Institute of Traditional Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
推荐引用方式 GB/T 7714	Wang, Zi-Yan,Hu, Zhi-Bi,Wang, Zheng-Tao. Regulation of Vitreoscilla hemoglobin on biosynthesis of astragaloside IV[J]. Yao xue xue bao = Acta pharmaceutica Sinica,2011,46(3):355-60.
APA	Wang, Zi-Yan,Hu, Zhi-Bi,&Wang, Zheng-Tao.(2011).Regulation of Vitreoscilla hemoglobin on biosynthesis of astragaloside IV.Yao xue xue bao = Acta pharmaceutica Sinica,46(3),355-60.
MLA	Wang, Zi-Yan,et al."Regulation of Vitreoscilla hemoglobin on biosynthesis of astragaloside IV".Yao xue xue bao = Acta pharmaceutica Sinica 46.3(2011):355-60.