Quantitative determination of myricetin in rat plasma by ultra performance liquid chromatography tandem mass spectrometry and its absolute bioavailability

doi:10.1055/s-0033-1363220

	Quantitative determination of myricetin in rat plasma by ultra performance liquid chromatography tandem mass spectrometry and its absolute bioavailability
	Dang, Y 4; Lin, G 4; Xie, Y 4; Duan, J 3; Ma, P 2; Li, G 1; Ji, G 3
刊名	Drug research
	2014-10
卷号	64 期号:10 页码:516-22
ISSN号	2194-9387
DOI	10.1055/s-0033-1363220
文献子类	Article
英文摘要	Myricetin is a widely distributed bioactive flavonoid with scientific interest attributed to its anti-oxidant, antitumor, and anti-inflammatory properties. A specific and sensitive ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method has been developed and validated for identification and quantification of myricetin in rat plasma after oral and intravenous administrations. Kaempferol was used as an internal standard. Followed by β-glucuronidase and sulfatase hydrolysis and liquid-liquid extraction with ethyl acetate, the analytes were separated on an Acquity UPLC BEH C18 column (2.1×50 mm, 1.7 μm) and analyzed in the selected ion recording with a negative electrospray ionization mode. The developed method was validated for selectivity, accuracy, precision, linearity, recovery, stability and matrix effect. The assay was validated over a wide concentration range of 2-4,000 ng/mL. Intra- and inter-day precisions were all less than 13.49% and accuracy ranged from 95.75 to 109.80%. The present method was successfully applied to investigate a pharmacokinetic study of myricetin following intravenous and oral administrations to rats. The absolute bioavailability was found to be 9.62% and 9.74% at 2 oral doses (50 mg/kg and 100 mg/kg, respectively), which indicated myricetin was poorly absorbed after oral administration. To our knowledge, this is the first pharmacokinetic evaluation of myricetin as a single active pharmaceutical ingredient in preclinical studies.
语种	英语
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/266365]
专题	中国科学院上海药物研究所
作者单位	1.Pharmacy Department, Shanghai TCM-integrated Hospital, Shanghai, China 2.Global Pharmaceutical Research and Development, Hospira Inc., McPherson, KS, USA; 3.Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, China; 4.Research Center for Health and Nutrition, Shanghai University of Traditional Chinese Medicine, Shanghai, China;
推荐引用方式 GB/T 7714	Dang, Y,Lin, G,Xie, Y,et al. Quantitative determination of myricetin in rat plasma by ultra performance liquid chromatography tandem mass spectrometry and its absolute bioavailability[J]. Drug research,2014,64(10):516-22.
APA	Dang, Y.,Lin, G.,Xie, Y.,Duan, J.,Ma, P.,...&Ji, G.(2014).Quantitative determination of myricetin in rat plasma by ultra performance liquid chromatography tandem mass spectrometry and its absolute bioavailability.Drug research,64(10),516-22.
MLA	Dang, Y,et al."Quantitative determination of myricetin in rat plasma by ultra performance liquid chromatography tandem mass spectrometry and its absolute bioavailability".Drug research 64.10(2014):516-22.