Determination of serum catecholamine metabolites in neuroblastoma by high performance liquid chromatography with electrochemical detection

doi:10.1002/bmc.1130010103

	Determination of serum catecholamine metabolites in neuroblastoma by high performance liquid chromatography with electrochemical detection
	Tang, Q M; Xu, X R; Shi, Y Q; Jin, B X
刊名	Biomedical chromatography : BMC
	1986-02
卷号	1 期号:1 页码:7-11
ISSN号	0269-3879
DOI	10.1002/bmc.1130010103
文献子类	Article
英文摘要	A method for determining serum catecholamine metabolites such as vanillylmandelic acid (VMA), 3-methoxy-4-hydroxyphenyl glycol (MHPG) and homovanillic acid (HVA) in neuroblastoma by using high performance liquid chromatography and electrochemical detector is described. The separation of catecholamine metabolites was performed on a reverse phase column with an eluting system containing citric acid-potassium hydrogen phosphate buffer and methanol as the organic modifier. The experimental results showed that VMA and HVA levels in the serum of neuroblastoma patients were 15-30 times higher than that of the normal control group. The same phenomenon also occurred in patients with stage II neuroblastoma. Serum VMA, MHPG and HVA levels reduced to normal in patients suffering from neuroblastoma after surgery. Serum catecholamine metabolites analysed by using HPLC/ECD is more simple, sensitive and reliable than that by usual urine assay and might be used for the diagnosis of neuroblastoma even in early stage.
语种	英语
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/266281]
专题	中国科学院上海药物研究所
作者单位	Shanghai Institute of Materia Medica, Academia Sinica, China
推荐引用方式 GB/T 7714	Tang, Q M,Xu, X R,Shi, Y Q,et al. Determination of serum catecholamine metabolites in neuroblastoma by high performance liquid chromatography with electrochemical detection[J]. Biomedical chromatography : BMC,1986,1(1):7-11.
APA	Tang, Q M,Xu, X R,Shi, Y Q,&Jin, B X.(1986).Determination of serum catecholamine metabolites in neuroblastoma by high performance liquid chromatography with electrochemical detection.Biomedical chromatography : BMC,1(1),7-11.
MLA	Tang, Q M,et al."Determination of serum catecholamine metabolites in neuroblastoma by high performance liquid chromatography with electrochemical detection".Biomedical chromatography : BMC 1.1(1986):7-11.