Nanostructural Surfaces with Different Elastic Moduli Regulate the Immune Response by Stretching Macrophages

doi:10.1021/acs.nanolett.9b00237

	Nanostructural Surfaces with Different Elastic Moduli Regulate the Immune Response by Stretching Macrophages
	Chen, Lan; Wang, Donghui; Peng, Feng; Qiu, Jiajun; Ouyang, Liping; Qjao, Yuqin; Liu, Xuanyong
刊名	NANO LETTERS
	2019-06-01
卷号	19 期号:6 页码:3480
关键词	surface elastic modulus macrophage polarization immune response implants molecular mechanism
ISSN号	1530-6984
DOI	10.1021/acs.nanolett.9b00237
文献子类	Article
英文摘要	A proper immune response is key for the successful implantation of biomaterials, and designing and fabricating biomaterials to regulate immune responses is the future trend. In this work, three different nanostructures were constructed on the surface of titanium using a hydrothermal method, and through a series of in vitro and in vivo experiments, we found that the aspect ratio of nanostructures can affect the elastic modulus of a material surface and further regulate immune cell behaviors. This work demonstrates that nanostructures with a higher aspect ratio can endow a material surface with a lower elastic modulus, which was confirmed by experiments and theoretical analyses. The deflection of nanostructures under the cell adsorption force is a substantial factor in stretching macrophages to enhance cell adhesion and spreading, further inducing macrophage polarization toward the M1 phenotype and leading to intense immune responses. In contrast, a nanostructure with a lower aspect ratio on a material surface leads to a higher surface elastic modulus, making deflection of the material difficult and creating a surface that is not conducive to macrophage adhesion and spreading, thus reducing the immune response. Moreover, molecular biology experiments indicated that regulation of the immune response by the elastic modulus is primarily related to the NF-kappa B signaling pathway. These findings suggest that the immune response can be regulated by constructing nanostructural surfaces with the proper elastic modulus through their influence on cell adhesion and spreading, which provides new insights into the surface design of biomaterials.
WOS研究方向	Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
语种	英语
出版者	AMER CHEMICAL SOC
内容类型	期刊论文
源URL	[http://ir.sic.ac.cn/handle/331005/27072]
专题	中国科学院上海硅酸盐研究所
推荐引用方式 GB/T 7714	Chen, Lan,Wang, Donghui,Peng, Feng,et al. Nanostructural Surfaces with Different Elastic Moduli Regulate the Immune Response by Stretching Macrophages[J]. NANO LETTERS,2019,19(6):3480.
APA	Chen, Lan.,Wang, Donghui.,Peng, Feng.,Qiu, Jiajun.,Ouyang, Liping.,...&Liu, Xuanyong.(2019).Nanostructural Surfaces with Different Elastic Moduli Regulate the Immune Response by Stretching Macrophages.NANO LETTERS,19(6),3480.
MLA	Chen, Lan,et al."Nanostructural Surfaces with Different Elastic Moduli Regulate the Immune Response by Stretching Macrophages".NANO LETTERS 19.6(2019):3480.