Minocycline hydrochloride loaded graphene oxide enables enhanced osteogenic activity in the presence of Gram-positive bacteria, Staphylococcus aureus

doi:10.1039/c9tb00405j

	Minocycline hydrochloride loaded graphene oxide enables enhanced osteogenic activity in the presence of Gram-positive bacteria, Staphylococcus aureus
	Qiu, Jiajun; Qian, Wenhao; Zhang, Jinkai; Chen, Dafu; Yeung, Kelvin W. K.; Liu, Xuanyong
刊名	JOURNAL OF MATERIALS CHEMISTRY B
	2019-06-14
卷号	7 期号:22 页码:3590
ISSN号	2050-750X
DOI	10.1039/c9tb00405j
文献子类	Article
英文摘要	Implant failures still happen because of bacterial infections and inferior osteogenic properties. Graphene oxide (GO) could induce osteogenic differentiation as well as exhibit superior antibacterial activity which may provide an answer for this issue. In this work, GO films loaded with minocycline hydrochloride (MH) were fabricated on titanium implant surfaces, and the antibacterial activity and osteogenic properties were studied in the presence of S. aureus and rat bone mesenchymal stem cells (rBMSCs) in in vitro and in vivo environments, respectively. The results indicated that the MH loaded GO films on titanium surfaces (MH&GO@Ti) exhibited enhanced osteogenic activity in vitro with improved alkaline phosphatase (ALP) activity and enhanced osteogenic related gene expressions including ALP, collagen-I, osteocalcin, and osteopontin. The in vitro results of the co-culture of rBMSCs and S. aureus indicated that the rBMSCs grew well on the MH&GO@Ti sample with a higher coverage of cells and the bacteria were almost unobservable on the surface. In the in vivo experiments, the MH&GO@Ti exhibited excellent antibacterial and osteogenic activities in the presence of bacteria. No inflammatory cells for example neutrophils were found and superior bone-implant integration was obtained. This study offers a new proposal for the clinical application of drug-loaded graphene coating.
WOS研究方向	Materials Science
语种	英语
出版者	ROYAL SOC CHEMISTRY
内容类型	期刊论文
源URL	[http://ir.sic.ac.cn/handle/331005/27051]
专题	中国科学院上海硅酸盐研究所
推荐引用方式 GB/T 7714	Qiu, Jiajun,Qian, Wenhao,Zhang, Jinkai,et al. Minocycline hydrochloride loaded graphene oxide enables enhanced osteogenic activity in the presence of Gram-positive bacteria, Staphylococcus aureus[J]. JOURNAL OF MATERIALS CHEMISTRY B,2019,7(22):3590.
APA	Qiu, Jiajun,Qian, Wenhao,Zhang, Jinkai,Chen, Dafu,Yeung, Kelvin W. K.,&Liu, Xuanyong.(2019).Minocycline hydrochloride loaded graphene oxide enables enhanced osteogenic activity in the presence of Gram-positive bacteria, Staphylococcus aureus.JOURNAL OF MATERIALS CHEMISTRY B,7(22),3590.
MLA	Qiu, Jiajun,et al."Minocycline hydrochloride loaded graphene oxide enables enhanced osteogenic activity in the presence of Gram-positive bacteria, Staphylococcus aureus".JOURNAL OF MATERIALS CHEMISTRY B 7.22(2019):3590.