Effects of Qijian mixture on type 2 diabetes assessed by metabonomics, gut microbiota and network pharmacology

doi:10.1016/j.phrs.2018.01.011

	Effects of Qijian mixture on type 2 diabetes assessed by metabonomics, gut microbiota and network pharmacology
	Gao, Kuo 5; Yang, Ran 4; Zhang, Pan 5; Wang, Zhiyong 3; Jia, Caixia 5; Zhang, Feilong 5; Li, Shaojing 2; Wang, Jinping 5; Murtaza, Ghulam 1,5; Xie, Hua 5
刊名	PHARMACOLOGICAL RESEARCH
	2018-04-01
卷号	130 页码:93-109
关键词	Type 2 diabetes Mechanism of action Molecular targets Metabonomics Gut microbiota System pharmacology
ISSN号	1043-6618
DOI	10.1016/j.phrs.2018.01.011
通讯作者	Zhao, Huihui(hh686@126.com) ; Wang, Wei(wangwei@bucm.edu.cn) ; Chen, Jianxin(CJX@bucm.edu.cn)
英文摘要	Qjjian mixture, a new traditional Chinese medicine (TCM) formula comprising of Astragalus membranaceus, Romulus euonymi, Coptis chinensis and Pueraria lobata, was designed to ameliorate the type 2 diabetes (T2D), and its safety and efficacy were evaluated in the research by metabonomics, gut micro biota and system pharmacology. To study the hypoglycemic effect of Qijian mixture, male KKay mice (28-30g, 8-9 week) and C57/BL6 mice (18-19 g, 8-9 week) were used. Thirty KKay diabetic mice were randomly distributed into 5 groups, abbreviated as Model group (Model), Low Qijian Mixture group (QJM(L)), High Qijian Mixture group (QJM(H)), Chinese Medicine (Gegen Qinlian Decoction) Positive group (GGQL), and Western Medicine (Metformin hydrochloride) Positive group (Metformin). C57/BL6 was considered as the healthy control group (Control). Moreover, a system pharmacology approach was utilized to assess the physiological targets involved in the action of Qijian mixture. There was no adverse drug reaction of Qijian mixture in the acute toxicity study and HE result, and, compared with Model group, Qijian mixture could modulate blood glycemic level safely and effectively. Callan Mixture was lesser effective than metformin hydrochloride; however, both showed similar hypoglycemic trend. Based on H-1 NMR based metabonomics study, the profoundly altered metabolites in Qijian mixture treatment group were identified. Qjjian mixture-related 55 proteins and 4 signaling pathways, including galactose metabolism, valine, leucine and isoleucine degradation metabolism, aminoacyl-tRNA biosynthesis metabolism and alanine, aspartate and glutamate metabolism pathways, were explored. The PCoA analysis of gut micro biota revealed that Qijian mixture treatment profoundly enriched bacteroidetes. In addition, the system pharmacology paradigm revealed that Qijian mixture acted through TP53, AKT1 and PPARA proteins. It was concluded that Qjjian mixture effectively alleviated T2D, and this effect was linked with the altered features of the metabolite profiles and the gut microbiota. (C) 2018 Elsevier Ltd. All rights reserved.
资助项目	National Natural Science Foundation of China[81522051] ; National Natural Science Foundation of China[81470191] ; National Natural Science Foundation of China[81530100] ; National Natural Science Foundation of China[81403368] ; Postgraduate Project of Beijing University of Chinese Medicine[2015-JYB-XS044] ; Postgraduate Project of Beijing University of Chinese Medicine[2016-JYB-XS007] ; Innovation Team of Wei Wang in Beijing University of Chinese Medicine ; Combined Traditional Chinese and Western Medicine Pharmacology (13 Common Construction)
WOS关键词	INSULIN-RESISTANCE ; METABOLIC SYNDROME ; ESCHERICHIA-COLI ; MICE ; OBESITY ; CELLS ; INFLAMMATION ; ENDOTOXEMIA ; PREVALENCE ; BERBERINE
WOS研究方向	Pharmacology & Pharmacy
语种	英语
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
WOS记录号	WOS:000433016900009
资助机构	National Natural Science Foundation of China ; Postgraduate Project of Beijing University of Chinese Medicine ; Innovation Team of Wei Wang in Beijing University of Chinese Medicine ; Combined Traditional Chinese and Western Medicine Pharmacology (13 Common Construction)
内容类型	期刊论文
源URL	[http://ir.ia.ac.cn/handle/173211/28188]
专题	中国科学院自动化研究所
通讯作者	Zhao, Huihui; Wang, Wei; Chen, Jianxin
作者单位	1.Chinese Acad Sci, Inst Automat, Beijing 100029, Peoples R China 2.China Acad Chinese Med Sci, Inst Chinese Mat Med, Beijing 100700, Peoples R China 3.FengNing Chinese Med Hosp, Xin Feng North Rd, Fengning 068350, Peoples R China 4.China Acad Chinese Med Sci, Guanganmen Hosp, Beijing 100053, Peoples R China 5.Beijing Univ Chinese Med, Bei San Huan East Rd, Beijing 100029, Peoples R China
推荐引用方式 GB/T 7714	Gao, Kuo,Yang, Ran,Zhang, Pan,et al. Effects of Qijian mixture on type 2 diabetes assessed by metabonomics, gut microbiota and network pharmacology[J]. PHARMACOLOGICAL RESEARCH,2018,130:93-109.
APA	Gao, Kuo.,Yang, Ran.,Zhang, Pan.,Wang, Zhiyong.,Jia, Caixia.,...&Chen, Jianxin.(2018).Effects of Qijian mixture on type 2 diabetes assessed by metabonomics, gut microbiota and network pharmacology.PHARMACOLOGICAL RESEARCH,130,93-109.
MLA	Gao, Kuo,et al."Effects of Qijian mixture on type 2 diabetes assessed by metabonomics, gut microbiota and network pharmacology".PHARMACOLOGICAL RESEARCH 130(2018):93-109.