Variation in biochemical composition during encystment of the planktonic dinoflagellate Akashiwo sanguinea in N-limited cultures

doi:10.1007/s00227-019-3569-2

CORC > 海洋研究所 > 中国科学院海洋研究所 > 海洋生态与环境科学重点实验室

	Variation in biochemical composition during encystment of the planktonic dinoflagellate Akashiwo sanguinea in N-limited cultures
	Liu, Yun 2,3,4; Chen, Tiantian2,3,4 ; Song, Shuqun 2,3,4; Li, Caiwen 1,2,3,4
刊名	MARINE BIOLOGY
	2019-09-01
卷号	166 期号:9 页码:12
ISSN号	0025-3162
DOI	10.1007/s00227-019-3569-2
通讯作者	Li, Caiwen(cwli@qdio.ac.cn)
英文摘要	The planktonic dinoflagellate Akashiwo sanguinea is a commonly observed bloom-forming species in estuarine and coastal waters worldwide. Large scales of A. sanguinea bloom cause the mass mortality of fish, shellfish, and sea birds. The formation of resting cysts plays as a potential vector for its wide distribution and frequent recurrence of blooms; however, the biochemical characteristics of algal cells during their life cycle remain unclear. For the first time, the variations in cellular chlorophyll a (Chl a), protein, carbohydrate, and total lipid contents during encystment of A. sanguinea cultured in different nitrate concentrations, namely 0-, 25-, 50-, 100-, 200-, and 833-mu M N-added treatment, were studied in the present study. The results indicated that resting cysts were formed in all the N treatments, with the highest encystment ratio of 3.34 +/- 0.57% observed in the 100-mu M N-added treatment, and the lowest rate of 0.80 +/- 0.03% observed in the 883-mu M N-added treatment. The levels of the four biochemical components varied significantly during the encystment process. The Chl a and protein levels were significantly lower in newly formed cysts than in vegetative cells; however, continuous accumulation of carbohydrates and total lipids occurred with the algal growth, particularly of carbohydrates in the resting cysts, which was more than tenfold greater than those in the vegetative cells. Low initial N concentrations were more favorable for carbohydrate accumulation in cysts than high N concentrations. The accumulated components may play vital roles for the substance of A. sanguinea during resting stage. The results provided fundamental information for an improved understanding of the physiological response of A. sanguinea during encystment.
资助项目	National Natural Science Foundation of China (NSFC)[41506141] ; National Key R&D Program of China[2017YFC1404300] ; Youth Talent Support Program of the Laboratory for Marine Ecology and Environmental Science, Pilot National Laboratory for Marine Science and Technology (Qingdao)[LMEES-YTSP-2018-01-06] ; NSFC-Shandong Joint Fund for Marine Science Research Center[U1606404]
WOS研究方向	Marine & Freshwater Biology
语种	英语
出版者	SPRINGER HEIDELBERG
WOS记录号	WOS:000483689700002
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/162831]
专题	海洋研究所_海洋生态与环境科学重点实验室
通讯作者	Li, Caiwen
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Shandong, Peoples R China 3.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Ecol & Environm Sci, Qingdao 266237, Shandong, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Liu, Yun,Chen, Tiantian,Song, Shuqun,et al. Variation in biochemical composition during encystment of the planktonic dinoflagellate Akashiwo sanguinea in N-limited cultures[J]. MARINE BIOLOGY,2019,166(9):12.
APA	Liu, Yun,Chen, Tiantian,Song, Shuqun,&Li, Caiwen.(2019).Variation in biochemical composition during encystment of the planktonic dinoflagellate Akashiwo sanguinea in N-limited cultures.MARINE BIOLOGY,166(9),12.
MLA	Liu, Yun,et al."Variation in biochemical composition during encystment of the planktonic dinoflagellate Akashiwo sanguinea in N-limited cultures".MARINE BIOLOGY 166.9(2019):12.