Alleviated Inhibition of Single Enzyme in Confined and Crowded Environment

doi:10.1021/acs.jpclett.8b03736

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2011-2017年

	Alleviated Inhibition of Single Enzyme in Confined and Crowded Environment
	Gao, YJ ; Liu, XG ; Sun, LL ; Xu, Y ; Yang, SC ; Fan, CH ; Li, D
刊名	JOURNAL OF PHYSICAL CHEMISTRY LETTERS
	2019
卷号	10 期号:1 页码:82—89
关键词	HORSERADISH-PEROXIDASE TIME KINETICS PROTEINS DYNAMICS OXIDASE VOLUME
ISSN号	1948-7185
DOI	10.1021/acs.jpclett.8b03736
文献子类	期刊论文
英文摘要	Most proteins perform functions in intracellular milieu. The crowding, compartmentalized cytosol environment affects the protein structure, folding, conformational stability, substrate diffusion, and substrate-enzyme binding. Moreover, enzymes are available at single or very low copy numbers in a cell, and thus the conformation fluctuations of a single enzyme in a crowding environment could also greatly influence its kinetics. However, the crowding effect is poorly understood in the kinetical aspect of enzymatic reactions. In the present study, individual horseradish peroxidase (HRP) is encapsulated in a liposome containing crowding reagents as mimics of viscous cytosol. The confined crowding environment possesses a profound influence on both the catalytic activity and the product inhibition of enzymes. By analyzing the correlation between product generation and product inhibition, we find that the allosteric noncompetitive inhibition of HRP is alleviated in the crowded and confined milieu. Small-angle X-ray scattering experiments provide straightforward proofs of structural changes of enzymes in crowding environments, which are responsible for the reduced enzyme activity and increased enzyme-substrate affinity. We expect that this work may deepen the understanding of correlations between enzymatic conformations and activity performance in real cellular environments.
语种	英语
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/31462]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
作者单位	1.East China Normal Univ, Sch Chem & Mol Engn, Shanghai 200241, Peoples R China; 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; 3.Chinese Acad Sci, Shanghai Synchrotron Radiat Facil, Key Lab Interfacial Phys & Technol, Shanghai Inst Appl Phys,Div Phys Biol, Shanghai 201800, Peoples R China; 4.Natl Engn Res Ctr Nanotechnol, Shanghai 200241, Peoples R China 5.Case Western Reserve Univ, Dept Nutr, Cleveland, OH 44106 USA; 6.Case Western Reserve Univ, Ctr Prote, Cleveland, OH 44106 USA; 7.Chinese Acad Sci, Shanghai Synchrotron Radiat Facil, Key Lab Interfacial Phys & Technol, Shanghai Inst Appl Phys,Bioimaging Ctr, Shanghai 201800, Peoples R China; 8.Shanghai Jiao Tong Univ, Inst Mol Med, Renji Hosp, Sch Med, Shanghai 200240, Peoples R China; 9.Shanghai Jiao Tong Univ, Sch Chem & Chem Engn, Renji Hosp, Sch Med, Shanghai 200240, Peoples R China;
推荐引用方式 GB/T 7714	Gao, YJ,Liu, XG,Sun, LL,et al. Alleviated Inhibition of Single Enzyme in Confined and Crowded Environment[J]. JOURNAL OF PHYSICAL CHEMISTRY LETTERS,2019,10(1):82—89.
APA	Gao, YJ.,Liu, XG.,Sun, LL.,Xu, Y.,Yang, SC.,...&Li, D.(2019).Alleviated Inhibition of Single Enzyme in Confined and Crowded Environment.JOURNAL OF PHYSICAL CHEMISTRY LETTERS,10(1),82—89.
MLA	Gao, YJ,et al."Alleviated Inhibition of Single Enzyme in Confined and Crowded Environment".JOURNAL OF PHYSICAL CHEMISTRY LETTERS 10.1(2019):82—89.