Single-Cell Mobility Analysis of Metastatic Breast Cancer Cells

doi:10.1002/advs.201801158

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2011-2017年

	Single-Cell Mobility Analysis of Metastatic Breast Cancer Cells
	Zhuang, JL ; Wu, YJ ; Chen, L ; Liang, SP ; Wu, MH ; Zhou, LD ; Fan, CH ; Zhang, YQ
刊名	ADVANCED SCIENCE
	2018
卷号	5 期号:12 页码:-
关键词	CIRCULATING TUMOR-CELLS RNA-SEQ MIGRATION MOTILITY HETEROGENEITY PLATFORM ZC3H12A
ISSN号	2198-3844
DOI	10.1002/advs.201801158
文献子类	期刊论文
英文摘要	Efforts have been taken to enhance the study of single-cells, however, the task remains challenging because most previous investigations cannot exclude the interactions between single cells or separately retrieved cells with specificity for further analyses. Here, a single-cell mobility analysis platform (SCM-Chip) is developed that can not only real-time monitor single-cell migration in independent niches but can also selectively recover target cells one by one. The design of each channel with a single-cell capture unit and an outlet enables the system to place single cells in different isolated niches with fluidic capture and to respectively collect target cells based on mobilities. SCM-Chip characterization of breast cancer cells reveals the presence of high- and low-migratory populations. Whole-cell transcriptome analysis establishes that monocyte chemotactic protein induced protein 1 (MCPIP1) is related with cell mobility; cells with a high expression of MCPIP1 exhibit low mobility in vitro and metastasis in vivo. The SCM platform provides a generic tool for accurate single-cell isolation and differentiation that can be readily adapted for the study of cancer and drug development.
语种	英语
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/31109]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
作者单位	1.Sun Yat Sen Univ, Zhongshan Sch Med, Dept Immunol, 74 Zhongshan 2nd Rd, Guangzhou 510080, Guangdong, Peoples R China 2.Sun Yat Sen Univ, Sch Pharmaceut Sci, Guangzhou 510006, Guangdong, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Appl Phys, Phys Biol Lab, Shanghai 201800, Peoples R China 4.Cent S Univ, Xiangya Hosp, Dept Gen Surg, Changsha 410008, Hunan, Peoples R China
推荐引用方式 GB/T 7714	Zhuang, JL,Wu, YJ,Chen, L,et al. Single-Cell Mobility Analysis of Metastatic Breast Cancer Cells[J]. ADVANCED SCIENCE,2018,5(12):-.
APA	Zhuang, JL.,Wu, YJ.,Chen, L.,Liang, SP.,Wu, MH.,...&Zhang, YQ.(2018).Single-Cell Mobility Analysis of Metastatic Breast Cancer Cells.ADVANCED SCIENCE,5(12),-.
MLA	Zhuang, JL,et al."Single-Cell Mobility Analysis of Metastatic Breast Cancer Cells".ADVANCED SCIENCE 5.12(2018):-.