Sidelobe suppression in light-sheet fluorescence microscopy with Bessel beam plane illumination using subtractive imaging

doi:10.3788/COL201816.111801

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2011-2017年

	Sidelobe suppression in light-sheet fluorescence microscopy with Bessel beam plane illumination using subtractive imaging
	Deng, SH ; Xiao, YP ; Hu, J ; Chen, JF ; Wang, YH ; Liu, MP
刊名	CHINESE OPTICS LETTERS
	2018
卷号	16 期号:11 页码:-
关键词	RESOLUTION ENHANCEMENT DIFFRACTION PROPAGATION MEDIA
ISSN号	1671-7694
DOI	10.3788/COL201816.111801
文献子类	期刊论文
英文摘要	The fluorescence from the out-of-focus region excited by the sidelobes of a Bessel beam is the major concern for light-sheet fluorescence microscopy( LSFM) with Bessel beam plane illumination. Here, we propose a method of applying the subtractive imaging to overcome the limitation of the conventional LSFM with Bessel beam plane illumination. In the proposed method, the sample is imaged twice by line scanning using the extended solid Bessel beam and the ring-like Bessel beam. By subtracting between the two images with similar out-of-focus blur, the improved image quality with the suppression of the Bessel beam sidelobes and enhanced sectioning ability with improved contrast are demonstrated.
语种	英语
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/31102]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
作者单位	1.Nanchang Univ, Sch Informat Engn, Nanchang 330031, Jiangxi, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Appl Phys, Div Phys Biol, Shanghai 201800, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Opt & Fine Mech, Shanghai 201800, Peoples R China
推荐引用方式 GB/T 7714	Deng, SH,Xiao, YP,Hu, J,et al. Sidelobe suppression in light-sheet fluorescence microscopy with Bessel beam plane illumination using subtractive imaging[J]. CHINESE OPTICS LETTERS,2018,16(11):-.
APA	Deng, SH,Xiao, YP,Hu, J,Chen, JF,Wang, YH,&Liu, MP.(2018).Sidelobe suppression in light-sheet fluorescence microscopy with Bessel beam plane illumination using subtractive imaging.CHINESE OPTICS LETTERS,16(11),-.
MLA	Deng, SH,et al."Sidelobe suppression in light-sheet fluorescence microscopy with Bessel beam plane illumination using subtractive imaging".CHINESE OPTICS LETTERS 16.11(2018):-.