comparativestudyofthetransfectionefficiencyofcommonlyusedviralvectorsinrhesusmonkeymacacamulattabrains | |
Wu Shihao1; Liao Zhixing1; Rizak Joshua D1; Zheng Na1; Zhang Linheng1; Tang Hen1; He Xiaobin2; Wu Yang2; He Xiaping1; Yang Meifeng3 | |
刊名 | zoologicalresearch |
2017 | |
卷号 | 38期号:2页码:88 |
ISSN号 | 2095-8137 |
英文摘要 | Viral vector transfection systems are among the simplest of biological agents with the ability to transfer genes into the central nervous system. In brain research, a series of powerful and novel gene editing technologies are based on these systems. Although many viral vectors are used in rodents, their full application has been limited in non-human primates. To identify viral vectors that can stably and effectively express exogenous genes within nonhuman primates, eleven commonly used recombinant adeno-associated viral and lentiviral vectors, each carrying a gene to express green or red fluorescence, were injected into the parietal cortex of four rhesus monkeys. The expression of fluorescent cells was used to quantify transfection efficiency. Histological results revealed that recombinant adeno-associated viral vectors, especially the serotype 2/9 coupled with the cytomegalovirus, human synapsin I, or Ca~(2+)/calmodulin-dependent protein kinase II promoters, and lentiviral vector coupled with the human ubiquitin C promoter, induced higher expression of fluorescent cells, representing high transfection efficiency. This is the first comparison of transfection efficiencies of different viral vectors carrying different promoters and serotypes in non-human primates(NHPs). These results can be used as an aid to select optimal vectors to transfer exogenous genes into the central nervous system of non-human primates. |
语种 | 英语 |
内容类型 | 期刊论文 |
源URL | [http://ir.wipm.ac.cn/handle/112942/19208] |
专题 | 中国科学院武汉物理与数学研究所 |
作者单位 | 1.中国科学院昆明动物研究所 2.中国科学院武汉物理与数学研究所 3.昆明医科大学 |
推荐引用方式 GB/T 7714 | Wu Shihao,Liao Zhixing,Rizak Joshua D,et al. comparativestudyofthetransfectionefficiencyofcommonlyusedviralvectorsinrhesusmonkeymacacamulattabrains[J]. zoologicalresearch,2017,38(2):88. |
APA | Wu Shihao.,Liao Zhixing.,Rizak Joshua D.,Zheng Na.,Zhang Linheng.,...&Hu Xintian.(2017).comparativestudyofthetransfectionefficiencyofcommonlyusedviralvectorsinrhesusmonkeymacacamulattabrains.zoologicalresearch,38(2),88. |
MLA | Wu Shihao,et al."comparativestudyofthetransfectionefficiencyofcommonlyusedviralvectorsinrhesusmonkeymacacamulattabrains".zoologicalresearch 38.2(2017):88. |
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