Development of 20 chloroplast microsatellite primers in wuyao (Lindera aggregata, Lauraceae)

doi:10.1002/aps3.1213

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	Development of 20 chloroplast microsatellite primers in wuyao (Lindera aggregata, Lauraceae)
	Ye, Jun-Wei 1,2; Li, De-Zhu1
刊名	APPLICATIONS IN PLANT SCIENCES
	2019-08-01
卷号	7 期号:8 页码:4
关键词	chloroplast microsatellite Lauraceae Lindera aggregata wuyao
ISSN号	2168-0450
DOI	10.1002/aps3.1213
通讯作者	Li, De-Zhu(dzl@mail.kib.ac.cn)
英文摘要	Premise of the Study To investigate the population genetics and evolutionary history of wuyao (Lindera aggregata, Lauraceae) and other Lindera species, polymorphic chloroplast microsatellite (cpSSR) primers were developed. Methods and Results The complete chloroplast genome of L. glauca was used to design 74 primers for L. aggregata. Agarose gel electrophoresis and Sanger sequencing were used to select 20 polymorphic primers. These primers were then tested in 70 individuals from three populations of L. aggregata. The number of alleles ranged from two to four, and the unbiased haploid diversity index ranged from 0.457 to 0.685. Up to 17 primers successfully amplified in four other Lindera species: L. prattii, L. chunii, L. lungshengensis, and L. pulcherrima var. hemsleyana. Conclusions The 20 primers reported here can potentially be used for future genetic studies of L. aggregata and other Lindera species.
WOS研究方向	Plant Sciences
语种	英语
WOS记录号	WOS:000482942400003
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn/handle/151853/68820]
专题	昆明植物研究所_中国西南野生生物种质资源库
通讯作者	Li, De-Zhu
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Kunming 650201, Yunnan, Peoples R China 2.Shanghai Sci & Technol Museum, Shanghai Nat Hist Museum, Nat Hist Res Ctr, Shanghai 200127, Peoples R China
推荐引用方式 GB/T 7714	Ye, Jun-Wei,Li, De-Zhu. Development of 20 chloroplast microsatellite primers in wuyao (Lindera aggregata, Lauraceae)[J]. APPLICATIONS IN PLANT SCIENCES,2019,7(8):4.
APA	Ye, Jun-Wei,&Li, De-Zhu.(2019).Development of 20 chloroplast microsatellite primers in wuyao (Lindera aggregata, Lauraceae).APPLICATIONS IN PLANT SCIENCES,7(8),4.
MLA	Ye, Jun-Wei,et al."Development of 20 chloroplast microsatellite primers in wuyao (Lindera aggregata, Lauraceae)".APPLICATIONS IN PLANT SCIENCES 7.8(2019):4.