Tracking the southern river terrapin (Batagur affinis) through environmental DNA: prospects and challenges

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	Tracking the southern river terrapin (Batagur affinis) through environmental DNA: prospects and challenges
	Wilson JJ*1; Sing KW 2; Chen PN 3; Zieritz A 4; wilso04@gmail.com, johnjameswilson@qq.com
刊名	Mitochondrial DNA A DNA Mapp Seq Anal
	2017
卷号	期号:页码:1-5
关键词	Batagur Affinis Kemaman Malaysia Critically Endangered Species Distribution Ecology Environmental Dna Genetic Population Molecular Marker Southern River Terrapin Species Monitoring Turtle
英文摘要	Environmental DNA detection has emerged as a powerful tool to monitor aquatic species without the need for capture or visual identification and is particularly useful for rare or elusive species. Our objective was to develop an eDNA approach for detecting the southern river terrapin (Batagur affinis) in Malaysia. We designed species-specific primers for a fragment of B. affinis mtDNA and evaluated their effectiveness in silico, in vitro and in situ. The primers amplified 110 bp of the cytochrome b mtDNA sequence of B. affinis from aquarium water samples housing nine juvenile B. affinis. We also successfully detected B. affinis eDNA from river samples taken from a site where turtles were known to be in the vicinity. Prospects and challenges of using an eDNA approach to help determine the distribution of B. affinis, essential information for an effective conservation plan, are discussed.
语种	英语
资助机构	This study was supported by the Turtle Conservation Fund (awarded 2016) and the University of Nottingham Malaysia Small Grant Scheme (awarded 2015). ; This study was supported by the Turtle Conservation Fund (awarded 2016) and the University of Nottingham Malaysia Small Grant Scheme (awarded 2015). ; This study was supported by the Turtle Conservation Fund (awarded 2016) and the University of Nottingham Malaysia Small Grant Scheme (awarded 2015). ; This study was supported by the Turtle Conservation Fund (awarded 2016) and the University of Nottingham Malaysia Small Grant Scheme (awarded 2015).
内容类型	期刊论文
源URL	[http://159.226.149.26:8080/handle/152453/12038]
专题	昆明动物研究所_遗传资源与进化国家重点实验室
通讯作者	wilso04@gmail.com, johnjameswilson@qq.com
作者单位	1.International College Beijing, China Agricultural University, Beijing, P. R. China 2.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, P. R. China 3.Turtle Conservation Society of Malaysia, Kemaman, Malaysia 4.School of Environmental and Geographical Sciences, University of Nottingham Malaysia Campus, Semenyih, Malaysia
推荐引用方式 GB/T 7714	Wilson JJ,Sing KW,Chen PN,et al. Tracking the southern river terrapin (Batagur affinis) through environmental DNA: prospects and challenges[J]. Mitochondrial DNA A DNA Mapp Seq Anal,2017,(*):1-5.
APA	Wilson JJ,Sing KW,Chen PN,Zieritz A,&wilso04@gmail.com, johnjameswilson@qq.com.(2017).Tracking the southern river terrapin (Batagur affinis) through environmental DNA: prospects and challenges.Mitochondrial DNA A DNA Mapp Seq Anal,(*),1-5.
MLA	Wilson JJ,et al."Tracking the southern river terrapin (Batagur affinis) through environmental DNA: prospects and challenges".Mitochondrial DNA A DNA Mapp Seq Anal .*(2017):1-5.