Silver nanoparticle based label-free colorimetric immunosensor for rapid detection of neurogenin 1

	Silver nanoparticle based label-free colorimetric immunosensor for rapid detection of neurogenin 1
	Yuan Y ; Zhang J ; Zhang HC ; Yang XR
刊名	analyst
	2012
卷号	137 期号:2 页码:496-501
关键词	CRANIAL SENSORY GANGLIA GOLD NANOPARTICLES NEURONAL DIFFERENTIATION P19 CELLS IMMUNOASSAY ASSAY PRECURSORS EXPRESSION DNA
ISSN号	0003-2654
通讯作者	zhang hc
中文摘要	neurogenin 1 (ngn1), with the functions of controlling the differentiation of neurons, determining specific neuronal subtype, and inhibiting glial differentiation, is quantitatively detected for the first time. by using specifically modified silver nanoparticles (agnp) as the signaling element, a label-free, rapid and sensitive colorimetric immunoassay for the synthetic peptide fragment of ngn1 (amino acid sequence: aqddeqerrrrrgrtr) is reported. the detection procedure is based on an antiaggregation mechanism, by which ngn1 inhibits the aggregation of the probe in the presence of salt (naclo(4)). the anti-ngn1 antibody conjugated agnp (denoted as agnp-ab) is negatively charged, and mono-binding of the like-charged ngn1 to the probe will increase the surface charge density, hence enhancing the interparticular electrostatic repulsion. along with the increase of ngn1 concentration, the color of the solution varies from red to yellow, thereby developing a feasible approach for the detection of ngn1. using a uv/vis spectrophotometer, this assay exhibits a linear response range of two orders of magnitude, from 50 to 800 ng ml(-1), and a detection limit of 30 ng ml(-1). on the basis of these qualities, the antibody-conjugated agnp may become a useful tool for point-of-care diagnosis of ngn1 and such a method offers a new insight on the detection of the analogous antigen fragment as well.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000297998000032
公开日期	2013-09-03
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/48751]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Yuan Y,Zhang J,Zhang HC,et al. Silver nanoparticle based label-free colorimetric immunosensor for rapid detection of neurogenin 1[J]. analyst,2012,137(2):496-501.
APA	Yuan Y,Zhang J,Zhang HC,&Yang XR.(2012).Silver nanoparticle based label-free colorimetric immunosensor for rapid detection of neurogenin 1.analyst,137(2),496-501.
MLA	Yuan Y,et al."Silver nanoparticle based label-free colorimetric immunosensor for rapid detection of neurogenin 1".analyst 137.2(2012):496-501.