Toward Improvement of Erythromycin A Production in an Industrial Saccharopolyspora erythraea Strain via Facilitation of Genetic Manipulation with an Artificial attB Site for Specific Recombination | |
WU JIEQUN ; ZHANG QINGLIN ; DENG WEI ; QIAN JIANGCHAO ; ZHANG SILIANG ; Liu W(刘文) | |
刊名 | Appl. Environ. Microbiol. |
2011 | |
卷号 | 77期号:21页码:7508-7516 |
ISSN号 | 0099-2240 |
其他题名 | Toward Improvement of Erythromycin A Production in an Industrial Saccharopolyspora erythraea Strain via Facilitation of Genetic Manipulation with an Artificial attB Site for Specific Recombination |
通讯作者 | 刘文 |
英文摘要 | Large-scale production of erythromycin A (Er-A) relies on the organism Saccharopolyspora erythraea, in which lack of a typical attB site largely impedes the application of phage Phi C31 integrase-mediated recombination into site-specific engineering. We herein report construction of an artificial attB site in an industrial S. erythraea strain, HL3168 E3, in an effort to break the bottleneck previously encountered during genetic manipulation mainly from homologous or unpredictable nonspecific integration. Replacement of a cryptic gene, nrps1-1, with a cassette containing eight attB DNA sequences did not affect the high Er-producing ability, setting the stage for precisely engineering the industrial Er-producing strain for foreign DNA introduction with a reliable conjugation frequency. Transfer of either exogenous or endogenous genes of importance to Er-A biosynthesis, including the S-adenosylmethionine synthetase gene for positive regulation, vhb for increasing the oxygen supply, and two tailoring genes, eryK and eryG, for optimizing the biotransformation at the late stage, was achieved by taking advantage of this facility, allowing systematic improvement of Er-A production as well as elimination of the by-products Er-B and Er-C in fermentation. The strategy developed here can generally be applicable to other strains that lack the attB site. |
学科主题 | 生命有机化学 |
收录类别 | SCI |
原文出处 | http://dx.doi.org/10.1128/AEM.06034-11 |
语种 | 英语 |
WOS记录号 | WOS:000296568200009 |
公开日期 | 2013-08-23 |
内容类型 | 期刊论文 |
源URL | [http://202.127.28.38/handle/331003/28481] |
专题 | 上海有机化学研究所_生命有机化学国家重点实验室 |
推荐引用方式 GB/T 7714 | WU JIEQUN,ZHANG QINGLIN,DENG WEI,et al. Toward Improvement of Erythromycin A Production in an Industrial Saccharopolyspora erythraea Strain via Facilitation of Genetic Manipulation with an Artificial attB Site for Specific Recombination[J]. Appl. Environ. Microbiol.,2011,77(21):7508-7516. |
APA | WU JIEQUN,ZHANG QINGLIN,DENG WEI,QIAN JIANGCHAO,ZHANG SILIANG,&刘文.(2011).Toward Improvement of Erythromycin A Production in an Industrial Saccharopolyspora erythraea Strain via Facilitation of Genetic Manipulation with an Artificial attB Site for Specific Recombination.Appl. Environ. Microbiol.,77(21),7508-7516. |
MLA | WU JIEQUN,et al."Toward Improvement of Erythromycin A Production in an Industrial Saccharopolyspora erythraea Strain via Facilitation of Genetic Manipulation with an Artificial attB Site for Specific Recombination".Appl. Environ. Microbiol. 77.21(2011):7508-7516. |
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