purificationoftheatgrp7rrmdomainfromarabidopsisthalianaanditspreliminarystructureandbindinganalysis

	purificationoftheatgrp7rrmdomainfromarabidopsisthalianaanditspreliminarystructureandbindinganalysis
	Chi Xiujuan 4; Qiao Xiaoya 3; Liu Ying 2; Liu Huili 1; Chen Lei 1; Wang Jihui 4; Ai Xuanjun 3
刊名	波谱学杂志
	2019-01-01
卷号	36 期号:1 页码:1
ISSN号	1000-4556
英文摘要	The glycine-rich RNA-binding protein, AtGrp7, is a component of a negative feedback loop in the circadian clock regulation of Arabidopsis thaliana. In our initial purification trial of the tobacco etch virus (TEV)-cleaved AtGrp7 RNA recognition motif (RRM) domain with the regular protocol, mixed ultraviolet signals of the target proteins and contaminants were observed. A two-step denaturing-refolding protocol was then tested, trying to solve the problem of impurities. The structure of the AtGrp71-90 RRM domain was fully recovered by quick-dilution refolding, evidenced by the fingerprint 1H-15N HSQC spectrum and CS-Rosetta model structures. Isothermal titration calorimetry (ITC) and NMR titration experiments further confirmed that the RRM domain of AtGrp71-90 had proper functions with regards to RNA/DNA binding.
语种	英语
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/176756]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.中国科学院物理研究所 2.Division of Virology & Immunology, National Center for AIDS/STD Control and Prevention 3.中国科学院大连化学物理研究所 4.大连工业大学
推荐引用方式 GB/T 7714	Chi Xiujuan,Qiao Xiaoya,Liu Ying,et al. purificationoftheatgrp7rrmdomainfromarabidopsisthalianaanditspreliminarystructureandbindinganalysis[J]. 波谱学杂志,2019,36(1):1.
APA	Chi Xiujuan.,Qiao Xiaoya.,Liu Ying.,Liu Huili.,Chen Lei.,...&Ai Xuanjun.(2019).purificationoftheatgrp7rrmdomainfromarabidopsisthalianaanditspreliminarystructureandbindinganalysis.波谱学杂志,36(1),1.
MLA	Chi Xiujuan,et al."purificationoftheatgrp7rrmdomainfromarabidopsisthalianaanditspreliminarystructureandbindinganalysis".波谱学杂志 36.1(2019):1.