SIRT5 Promotes Hepatocellular Carcinoma Progression by Regulating Mitochondrial Apoptosis

doi:10.7150/jca.31266

	SIRT5 Promotes Hepatocellular Carcinoma Progression by Regulating Mitochondrial Apoptosis
	Zhang, Rixin 3; Wang, Chengye 3; Tian, Yu 2,3; Yao, Yifan 3; Mao, Jiakai 3; Wang, Haibo 3; Li, Zhenghan 3; Xu, Yakun 3; Ye, Mingliang 1; Wang, Liming 3
刊名	JOURNAL OF CANCER
	2019
卷号	10 期号:16 页码:3871-3882
关键词	SIRT5 HCC Cytochrome c Acetylation
ISSN号	1837-9664
DOI	10.7150/jca.31266
通讯作者	Wang, Liming(wangbcc259@163.com)
英文摘要	SIRT5 belongs to a family of NAD(+)-dependent lysine deacetylases called sirtuins. Although accumulating evidence indicates SIRT5 upregulation in cancers, including liver cancer, the detailed roles and mechanisms remain to be revealed. Hepatocellular carcinoma (HCC) is the second leading cause of cancer-related deaths among men worldwide, and finding effective targets for HCC treatment and prevention is urgently needed. In the present study, we confirmed that mitochondrial sirtuins, particularly SIRT5, are more highly expressed in HCC cell lines than in normal liver cell lines. Moreover, SIRT5 knockdown suppresses HCC cell proliferation and SIRT5 overexpression promotes HCC cell proliferation. Furthermore, we verified that SIRT5 knockdown increases HCC cell apoptosis via the mitochondrial pathway. By co-IP and western blotting, we illustrated that SIRT5 deacetylates cytochrome c thus regulating HCC cell apoptosis. Taken together, our findings suggest that SIRT5 may function as a prognostic factor and drug target for HCC treatment.
资助项目	National Natural Science Foundation of China[81272368] ; National Natural Science Foundation of China[81471755] ; Clinical Capability Construction Project for Liaoning Provincial Hospitals[LNCCC-B03-2014]
WOS关键词	CANCER STATISTICS ; SIRTUINS ; DESUCCINYLATION ; METABOLISM ; PHYSIOLOGY ; REDUCTION ; PROTEINS
WOS研究方向	Oncology
语种	英语
出版者	IVYSPRING INT PUBL
WOS记录号	WOS:000471273000030
资助机构	National Natural Science Foundation of China ; National Natural Science Foundation of China ; Clinical Capability Construction Project for Liaoning Provincial Hospitals ; Clinical Capability Construction Project for Liaoning Provincial Hospitals ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Clinical Capability Construction Project for Liaoning Provincial Hospitals ; Clinical Capability Construction Project for Liaoning Provincial Hospitals ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Clinical Capability Construction Project for Liaoning Provincial Hospitals ; Clinical Capability Construction Project for Liaoning Provincial Hospitals ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Clinical Capability Construction Project for Liaoning Provincial Hospitals ; Clinical Capability Construction Project for Liaoning Provincial Hospitals
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/176147]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Wang, Liming
作者单位	1.Chinese Acad Sci, Natl Chromatog Res & Anal Ctr, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, 457 Zhongshan Rd, Dalian 116023, Liaoning, Peoples R China 2.Dalian Med Univ, Dept Vasc Surg, Hosp 2, 467 Zhongshan Rd, Dalian 116023, Liaoning, Peoples R China 3.Dalian Med Univ, Dept Surg, Div Hepatobiliary & Pancreat Surg, Hosp 2, 467 Zhongshan Rd, Dalian 116023, Liaoning, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Rixin,Wang, Chengye,Tian, Yu,et al. SIRT5 Promotes Hepatocellular Carcinoma Progression by Regulating Mitochondrial Apoptosis[J]. JOURNAL OF CANCER,2019,10(16):3871-3882.
APA	Zhang, Rixin.,Wang, Chengye.,Tian, Yu.,Yao, Yifan.,Mao, Jiakai.,...&Wang, Liming.(2019).SIRT5 Promotes Hepatocellular Carcinoma Progression by Regulating Mitochondrial Apoptosis.JOURNAL OF CANCER,10(16),3871-3882.
MLA	Zhang, Rixin,et al."SIRT5 Promotes Hepatocellular Carcinoma Progression by Regulating Mitochondrial Apoptosis".JOURNAL OF CANCER 10.16(2019):3871-3882.