Structural Analysis of Gangliosides Based on Electrospray Quadrupole Time-of-Flight Mass Spectrometry

doi:10.19756/j.issn.0253-3820.181804

	Structural Analysis of Gangliosides Based on Electrospray Quadrupole Time-of-Flight Mass Spectrometry
	Zhang Hua-Lin 4; Guo Zhi-Mou 2,3; Wang Lian-Zhi 4; Jin Gao-Wa 2,3; Zou Li-Hong 1; Lyu Yuan-Yuan 1; Ma Ming-Hui 1; Yan Jing-Yu 2,3; Duan Zheng-Chao 4; Liang Xin-Miao 2,3
刊名	CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
	2019-06-01
卷号	47 期号:6 页码:925-932
关键词	Ganglioside Electrospray-quadrupole-time-of-flight mass spectrometry Structural analysis
ISSN号	0253-3820
DOI	10.19756/j.issn.0253-3820.181804
通讯作者	Yan Jing-Yu(yanjingyu@dicp.ac.cn) ; Duan Zheng-Chao()
英文摘要	Gangliosides are glycosphingolipid compounds containing sialic acid, which are natural components of vertebrate cell membranes. As a clinical drug, gangliosides are widely used in the treatment of hypoxic ischemic encephalopathy, central nervous system injury, neurodegenerative diseases and peripheral neuropathy. The structural identification of gangliosides helps to understand the composition of the drug and improve the quality of the drug. In this work, an electrospray ionization quadrupole time-of-flight mass spectrometry ( ESI-Q-TOF-MS ) method for analysis of ganglioside structure was established. The optimization of the mass spectrometry parameters were established based on testing of two gangliosides controls. The mass spectrometry conditions were established (positive mode , capillary voltage of 3500 V, fragmentor voltage of 200 V, and collision energy of 40 eV) to obtain the fragment structure of the sugar chain. The fragmentation structure of ceramide was obtained with collision energy of 80 eV. The method was applied to the structural analysis of 9 gangliosides prepared in the laboratory. The samples were categorized and classified by comparison with molecular weights of the reference substances. Detailed structure analysis was performed based on the characteristic fragments in the secondary mass spectra of the samples. The results of this study indicated that the method could be applied to the mass spectrometric identification of various gangliosides, laying a foundation for more complex ganglioside mass spectrometry analysis.
资助项目	National Natural Science Foundation of China[21505131]
WOS关键词	LIQUID-CHROMATOGRAPHY ; IDENTIFICATION ; SEPARATION ; CERAMIDES ; COMPLEX ; CHIP
WOS研究方向	Chemistry
语种	英语
出版者	SCIENCE PRESS
WOS记录号	WOS:000470983800019
资助机构	National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/176132]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Yan Jing-Yu; Duan Zheng-Chao
作者单位	1.Qilu Pharmaceut Co Ltd, Jinan 250101, Shandong, Peoples R China 2.DICP CMC Innovat Inst Med, Taizhou 225300, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 4.Hubei Univ Nationalities, Enshi 445000, Peoples R China
推荐引用方式 GB/T 7714	Zhang Hua-Lin,Guo Zhi-Mou,Wang Lian-Zhi,et al. Structural Analysis of Gangliosides Based on Electrospray Quadrupole Time-of-Flight Mass Spectrometry[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2019,47(6):925-932.
APA	Zhang Hua-Lin.,Guo Zhi-Mou.,Wang Lian-Zhi.,Jin Gao-Wa.,Zou Li-Hong.,...&Liang Xin-Miao.(2019).Structural Analysis of Gangliosides Based on Electrospray Quadrupole Time-of-Flight Mass Spectrometry.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,47(6),925-932.
MLA	Zhang Hua-Lin,et al."Structural Analysis of Gangliosides Based on Electrospray Quadrupole Time-of-Flight Mass Spectrometry".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 47.6(2019):925-932.